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A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis

2013-03Journal article Open access
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dc.contributor.authorRemy, E.
dc.contributor.authorCabrito, T. R.
dc.contributor.authorBaster, P.
dc.contributor.authorBatista, R. A.
dc.contributor.authorTeixeira, M. C.
dc.contributor.authorFriml, J.
dc.contributor.authorSa-Correia, I.
dc.contributor.authorDuque, P.
dc.date.accessioned2016-04-26T16:45:04Z
dc.date.available2016-04-26T16:45:04Z
dc.date.issued2013-03
dc.description.abstractMany key aspects of plant development are regulated by the polarized transport of the phytohormone auxin. Cellular auxin efflux, the rate-limiting step in this process, has been shown to rely on the coordinated action of PIN-formed (PIN) and B-type ATP binding cassette (ABCB) carriers. Here, we report that polar auxin transport in the Arabidopsis thaliana root also requires the action of a Major Facilitator Superfamily (MFS) transporter, Zinc-Induced Facilitator-Like 1 (ZIFL1). Sequencing, promoter-reporter, and fluorescent protein fusion experiments indicate that the full-length ZIFL1.1 protein and a truncated splice isoform, ZIFL1.3, localize to the tonoplast of root cells and the plasma membrane of leaf stomatal guard cells, respectively. Using reverse genetics, we show that the ZIFL1.1 transporter regulates various root auxin-related processes, while the ZIFL1.3 isoform mediates drought tolerance by regulating stomatal closure. Auxin transport and immunolocalization assays demonstrate that ZIFL1.1 indirectly modulates cellular auxin efflux during shootward auxin transport at the root tip, likely by regulating plasma membrane PIN2 abundance. Finally, heterologous expression in yeast revealed that ZIFL1.1 and ZIFL1.3 share H(+)-coupled K(+) transport activity. Thus, by determining the subcellular and tissue distribution of two isoforms, alternative splicing dictates a dual function for the ZIFL1 transporter. We propose that this MFS carrier regulates stomatal movements and polar auxin transport by modulating potassium and proton fluxes in Arabidopsis cells.pt_PT
dc.description.sponsorshipFCT fellowships: (SFRH/BPD/44640/2008, SFRH/BPD/81221/2011).pt_PT
dc.identifier.citationRemy, E., Cabrito, T. R., Baster, P., Batista, R. A., Teixeira, M. C., Friml, J., Sá-Correia, I., Duque, P. (2013). A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis. Plant Cell, 25(3), 901–926.pt_PT
dc.identifier.doi10.1105/tpc.113.110353pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/586
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society of Plant Biologistspt_PT
dc.relationHerbicide resistance in Arabidopsis thaliana: Role of plant multidrug resistance transporters
dc.relation.publisherversionhttp://www.plantcell.org/content/25/3/901.long#cited-bypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAlternative Splicingpt_PT
dc.subjectArabidopsispt_PT
dc.subjectArabidopsis Proteinspt_PT
dc.subjectBiological Transportpt_PT
dc.subjectCell Membranept_PT
dc.subjectGene Expression Regulation, Plantpt_PT
dc.subjectIndoleacetic Acidspt_PT
dc.subjectMeristempt_PT
dc.subjectPlant Stomatapt_PT
dc.subjectPlant Transpirationpt_PT
dc.subjectPromoter Regions, Geneticpt_PT
dc.subjectProtein Isoformspt_PT
dc.subjectRecombinant Fusion Proteinspt_PT
dc.subjectReverse Geneticspt_PT
dc.subjectAdaptation, Biologicalpt_PT
dc.subjectDroughtspt_PT
dc.subjectGenes, Plantpt_PT
dc.subjectStress, Physiologicalpt_PT
dc.titleA Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHerbicide resistance in Arabidopsis thaliana: Role of plant multidrug resistance transporters
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F67858%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F102967%2F2008/PT
oaire.citation.endPage926pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage901pt_PT
oaire.citation.titleThe Plant Cellpt_PT
oaire.citation.volume25pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication78f7c49c-638f-40bd-94b8-3a4eb352d61a
relation.isProjectOfPublication87d8831f-5f09-40d2-bff7-79c374b5f000
relation.isProjectOfPublication.latestForDiscovery78f7c49c-638f-40bd-94b8-3a4eb352d61a

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