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REST is a hypoxia-responsive transcriptional repressor

2016-08-17Journal article Open access
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dc.contributor.authorCavadas, Miguel A. S.
dc.contributor.authorMesnieres, Marion
dc.contributor.authorCrifo, Bianca
dc.contributor.authorManresa, Mario C.
dc.contributor.authorSelfridge, Andrew C.
dc.contributor.authorKeogh, Ciara E.
dc.contributor.authorFabian, Zsolt
dc.contributor.authorScholz, Carsten C.
dc.contributor.authorNolan, Karen A.
dc.contributor.authorRocha, Liliane M. A.
dc.contributor.authorTambuwala, Murtaza M.
dc.contributor.authorBrown, Stuart
dc.contributor.authorWdowicz, Anita
dc.contributor.authorCorbett, Danielle
dc.contributor.authorMurphy, Keith J.
dc.contributor.authorGodson, Catherine
dc.contributor.authorCummins, Eoin P.
dc.contributor.authorTaylor, Cormac T.
dc.contributor.authorCheong, Alex
dc.date.accessioned2016-10-18T14:21:34Z
dc.date.available2016-10-18T14:21:34Z
dc.date.issued2016-08-17
dc.description.abstractCellular exposure to hypoxia results in altered gene expression in a range of physiologic and pathophysiologic states. Discrete cohorts of genes can be either up- or down-regulated in response to hypoxia. While the Hypoxia-Inducible Factor (HIF) is the primary driver of hypoxia-induced adaptive gene expression, less is known about the signalling mechanisms regulating hypoxia-dependent gene repression. Using RNA-seq, we demonstrate that equivalent numbers of genes are induced and repressed in human embryonic kidney (HEK293) cells. We demonstrate that nuclear localization of the Repressor Element 1-Silencing Transcription factor (REST) is induced in hypoxia and that REST is responsible for regulating approximately 20% of the hypoxia-repressed genes. Using chromatin immunoprecipitation assays we demonstrate that REST-dependent gene repression is at least in part mediated by direct binding to the promoters of target genes. Based on these data, we propose that REST is a key mediator of gene repression in hypoxia.pt_PT
dc.description.sponsorshipScience Foundation Ireland grant: (06/CE/B1129).pt_PT
dc.identifier.citationCavadas, M. A. S. et al. REST is a hypoxia-responsive transcriptional repressor. Sci. Rep. 6, 31355; doi: 10.1038/srep31355 (2016).pt_PT
dc.identifier.doi10.1038/srep31355pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/702
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relation.publisherversionhttp://www.nature.com/articles/srep31355pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGene silencingpt_PT
dc.subjectTranscriptional regulatory elementspt_PT
dc.titleREST is a hypoxia-responsive transcriptional repressorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage13pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume6pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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