Repository logo
 
Publication

Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis

2013-01-22Journal article Open access
Simple item page
dc.contributor.authorEichinger, Christian S
dc.contributor.authorKurze, Alexander
dc.contributor.authorOliveira, Raquel A
dc.contributor.authorNasmyth, Kim
dc.date.accessioned2016-06-27T16:30:28Z
dc.date.available2016-06-27T16:30:28Z
dc.date.issued2013-01-22
dc.description.abstractCohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct.pt_PT
dc.description.sponsorshipFP7 Marie Curie Intra‐European Fellowship; ERC grant.pt_PT
dc.identifier.citationEichinger, C. S., Kurze, A., Oliveira, R. A., Nasmyth, K. (2013). Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis. The EMBO Journal, 32(5), 656–665.pt_PT
dc.identifier.doi10.1038/emboj.2012.346pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/665
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherEmbo Presspt_PT
dc.relation.publisherversionhttp://emboj.embopress.org/content/32/5/656.longpt_PT
dc.subjectAnimalspt_PT
dc.subjectAnimals, Genetically Modifiedpt_PT
dc.subjectBlotting, Westernpt_PT
dc.subjectCell Cycle Proteinspt_PT
dc.subjectChromatinpt_PT
dc.subjectChromosomal Proteins, Non-Histonept_PT
dc.subjectDNApt_PT
dc.subjectDrosophila Proteinspt_PT
dc.subjectDrosophila melanogasterpt_PT
dc.subjectFemalept_PT
dc.subjectImmunoenzyme Techniquespt_PT
dc.subjectInterphasept_PT
dc.subjectMalept_PT
dc.subjectMitosispt_PT
dc.subjectNeuronspt_PT
dc.subjectPolytene Chromosomespt_PT
dc.subjectRNA, Messengerpt_PT
dc.subjectReal-Time Polymerase Chain Reactionpt_PT
dc.subjectReverse Transcriptase Polymerase Chain Reactionpt_PT
dc.subjectSalivary Glandspt_PT
dc.titleDisengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage665pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage656pt_PT
oaire.citation.titleThe EMBO Journalpt_PT
oaire.citation.volume32pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Disengaging the Smc3.pdf
Size:
2.68 MB
Format:
Adobe Portable Document Format
Description:
artigo principal
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CD- Artigos