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The Impact of Host Diet on Wolbachia Titer in Drosophila

2015-03-31Journal article Open access
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dc.contributor.authorSerbus, Laura R.
dc.contributor.authorWhite, Pamela M.
dc.contributor.authorSilva, Jessica Pintado
dc.contributor.authorRabe, Amanda
dc.contributor.authorTeixeira, Luis
dc.contributor.authorAlbertson, Roger
dc.contributor.authorSullivan, William
dc.date.accessioned2015-10-15T14:58:06Z
dc.date.available2015-10-15T14:58:06Z
dc.date.issued2015-03-31
dc.description.abstractWhile a number of studies have identified host factors that influence endosymbiont titer, little is known concerning environmental influences on titer. Here we examined nutrient impact on maternally transmitted Wolbachia endosymbionts in Drosophila. We demonstrate that Drosophila reared on sucrose- and yeast-enriched diets exhibit increased and reduced Wolbachia titers in oogenesis, respectively. The yeast-induced Wolbachia depletion is mediated in large part by the somatic TOR and insulin signaling pathways. Disrupting TORC1 with the small molecule rapamycin dramatically increases oocyte Wolbachia titer, whereas hyper-activating somatic TORC1 suppresses oocyte titer. Furthermore, genetic ablation of insulin-producing cells located in the Drosophila brain abolished the yeast impact on oocyte titer. Exposure to yeast-enriched diets altered Wolbachia nucleoid morphology in oogenesis. Furthermore, dietary yeast increased somatic Wolbachia titer overall, though not in the central nervous system. These findings highlight the interactions between Wolbachia and germline cells as strongly nutrient-sensitive, and implicate conserved host signaling pathways by which nutrients influence Wolbachia titer.pt_PT
dc.description.sponsorshipFlorida International University, National Institutes of Health, Minority Access to Research Careers program: (GMS-2T34GM007910), National Science Foundation grant: (MCB-1122252).pt_PT
dc.identifier10.1371/journal.ppat.1004777
dc.identifier.citationSerbus LR, White PM, Silva JP, Rabe A, Teixeira L, Albertson R, et al. (2015) The Impact of Host Diet on Wolbachia Titer in Drosophila . PLoS Pathog 11(3): e1004777. doi:10.1371/journal. ppat.1004777pt_PT
dc.identifier.doi10.1371/journal.ppat.1004777
dc.identifier.doi10.1371/journal.ppat.1004777
dc.identifier.urihttp://hdl.handle.net/10400.7/404
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relation.publisherversionhttp://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1004777pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWolbachiapt_PT
dc.subjectDrosophilapt_PT
dc.titleThe Impact of Host Diet on Wolbachia Titer in Drosophilapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage25pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePlos Pathogenspt_PT
oaire.citation.volume11pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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