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Plasma membrane H+-ATPases sustain pollen tube growth and fertilization

2020Journal article Open access
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dc.contributor.authorHoffmann, Robert D.
dc.contributor.authorPortes, Maria Teresa
dc.contributor.authorOlsen, Lene Irene
dc.contributor.authorDamineli, Daniel Santa Cruz
dc.contributor.authorHayashi, Maki
dc.contributor.authorNunes, Custódio O.
dc.contributor.authorPedersen, Jesper T.
dc.contributor.authorLima, Pedro T.
dc.contributor.authorCampos, Cláudia
dc.contributor.authorFeijó, José A.
dc.contributor.authorPalmgren, Michael
dc.date.accessioned2021-01-05T10:18:09Z
dc.date.available2021-01-05T10:18:09Z
dc.date.issued2020
dc.description.abstractPollen tubes are highly polarized tip-growing cells that depend on cytosolic pH gradients for signaling and growth. Autoinhibited plasma membrane proton (H+) ATPases (AHAs) have been proposed to energize pollen tube growth and underlie cell polarity, however, mechanistic evidence for this is lacking. Here we report that the combined loss of AHA6, AHA8, and AHA9 in Arabidopsis thaliana delays pollen germination and causes pollen tube growth defects, leading to drastically reduced fertility. Pollen tubes of aha mutants had reduced extracellular proton (H+) and anion fluxes, reduced cytosolic pH, reduced tip-to-shank proton gradients, and defects in actin organization. Furthermore, mutant pollen tubes had less negative membrane potentials, substantiating a mechanistic role for AHAs in pollen tube growth through plasma membrane hyperpolarization. Our findings define AHAs as energy transducers that sustain the ionic circuit defining the spatial and temporal profiles of cytosolic pH, thereby controlling downstream pH-dependent mechanisms essential for pollen tube elongation, and thus plant fertility.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41467-020-16253-1pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/966
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectArabidopsispt_PT
dc.subjectArabidopsis Proteinspt_PT
dc.subjectCell Membranept_PT
dc.subjectCell Polaritypt_PT
dc.subjectCytosolpt_PT
dc.subjectGene Knockdown Techniquespt_PT
dc.subjectGerminationpt_PT
dc.subjectHydrogen-Ion Concentrationpt_PT
dc.subjectMembrane Potentialspt_PT
dc.subjectMutationpt_PT
dc.subjectPlants, Genetically Modifiedpt_PT
dc.subjectPollen Tubept_PT
dc.subjectPollinationpt_PT
dc.subjectProtein Isoformspt_PT
dc.subjectProton-Translocating ATPasespt_PT
dc.subjectSpatio-Temporal Analysispt_PT
dc.titlePlasma membrane H+-ATPases sustain pollen tube growth and fertilizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-PLA%2F4018%2F2012/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage2395pt_PT
oaire.citation.titleNature Communicationspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication8869b2a9-f7d7-4481-9d6b-9c18f6408fb4
relation.isProjectOfPublication.latestForDiscovery8869b2a9-f7d7-4481-9d6b-9c18f6408fb4

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