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Within host selection for faster replicating bacterial symbionts

2018-01-18Journal article Open access
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dc.contributor.authorChrostek, Ewa
dc.contributor.authorTeixeira, Luis
dc.date.accessioned2018-01-25T17:00:43Z
dc.date.available2018-01-25T17:00:43Z
dc.date.issued2018-01-18
dc.descriptionThis deposit is composed by the main article plus the supplementary materials of the publication.pt_PT
dc.descriptionThe supplementary materials are present in the publisher's page in the following link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191530#sec010pt_PT
dc.description.abstractWolbachia is a widespread, intracellular symbiont of arthropods, able to induce reproductive distortions and antiviral protection in insects. Wolbachia can also be pathogenic, as is the case with wMelPop, a virulent variant of the endosymbiont of Drosophila melanogaster. An extensive genomic amplification of the 20kb region encompassing eight Wolbachia genes, called Octomom, is responsible for wMelPop virulence. The Octomom copy number in wMelPop can be highly variable between individual D. melanogaster flies, even when comparing siblings arising from a single female. Moreover, Octomom copy number can change rapidly between generations. These data suggest an intra-host variability in Octomom copy number between Wolbachia cells. Since wMelPop Wolbachia with different Octomom copy numbers grow at different rates, we hypothesized that selection could act on this intra-host variability. Here we tested if total Octomom copy number changes during the lifespan of individual Drosophila hosts, revealing selection for different Wolbachia populations. We performed a time course analysis of Octomom amplification in flies whose mothers were controlled for Octomom copy number. We show that despite the Octomom copy number being relatively stable it increases slightly throughout D. melanogaster adult life. This indicates that there is selection acting on the intra-host variation in the Octomom copy number over the lifespan of individual hosts. This within host selection for faster replicating bacterial symbionts may be in conflict with between host selection against highly pathogenic Wolbachia.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia (www.fct.pt) grant PTDC/BEX-GMG/3128/2014; EMBO Long Term Fellowship: (EMBO ALTF 1497-2015); Marie Curie Actions; European Commission grants: (LTFCOFUND2013, GA-2013-609409).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationChrostek E, Teixeira L (2018) Within host selection for faster replicating bacterial symbionts. PLoS ONE 13(1): e0191530. https://doi.org/10.1371/journal.pone.0191530pt_PT
dc.identifier.doi10.1371/journal.pone.0191530pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/824
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relationEMBO ALTF 1497-2015pt_PT
dc.relationSupporting transnational mobility within the European life sciences by cofunding of the EMBO Fellowship Programme
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191530pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectWolbachiapt_PT
dc.subjectDrosophila melanogasterpt_PT
dc.subjectInvertebrate genomicspt_PT
dc.subjectNatural selectionpt_PT
dc.subjectBacterial pathogenspt_PT
dc.subjectGene poolpt_PT
dc.subjectGene amplificationpt_PT
dc.subjectPolymerase chain reactionpt_PT
dc.titleWithin host selection for faster replicating bacterial symbiontspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSupporting transnational mobility within the European life sciences by cofunding of the EMBO Fellowship Programme
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-GMG%2F3128%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/609409/EU
oaire.citation.endPage8pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePlos Onept_PT
oaire.citation.volume13pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationc4809a58-1420-4b1a-86f6-e84a0f1f41de
relation.isProjectOfPublicatione4e54767-b680-43d8-bada-ba0c909acfec
relation.isProjectOfPublication.latestForDiscoverye4e54767-b680-43d8-bada-ba0c909acfec

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