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Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression

2014-09-29Journal article Open access
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dc.contributor.authorCruz, Tiago
dc.contributor.authorCarvalho, Raquel
dc.contributor.authorRichardson, Dale
dc.contributor.authorDuque, Paula
dc.date.accessioned2015-10-01T15:23:27Z
dc.date.available2015-10-01T15:23:27Z
dc.date.issued2014-09-29
dc.description.abstractSerine/arginine-rich (SR) proteins are major modulators of alternative splicing, a key generator of proteomic diversity and flexible means of regulating gene expression likely to be crucial in plant environmental responses. Indeed, mounting evidence implicates splicing factors in signal transduction of the abscisic acid (ABA) phytohormone, which plays pivotal roles in the response to various abiotic stresses. Using real-time RT-qPCR, we analyzed total steady-state transcript levels of the 18 SR and two SR-like genes from Arabidopsis thaliana in seedlings treated with ABA and in genetic backgrounds with altered expression of the ABA-biosynthesis ABA2 and the ABA-signaling ABI1 and ABI4 genes. We also searched for ABA-responsive cis elements in the upstream regions of the 20 genes. We found that members of the plant-specific SC35-Like (SCL) Arabidopsis SR protein subfamily are distinctively responsive to exogenous ABA, while the expression of seven SR and SR-related genes is affected by alterations in key components of the ABA pathway. Finally, despite pervasiveness of established ABA-responsive promoter elements in Arabidopsis SR and SR-like genes, their expression is likely governed by additional, yet unidentified cis-acting elements. Overall, this study pinpoints SR34, SR34b, SCL30a, SCL28, SCL33, RS40, SR45 and SR45a as promising candidates for involvement in ABA-mediated stress responses.pt_PT
dc.description.sponsorshipFCT PostDoctoral Fellowships: SFRH/BPD/80073/2011, SFRH/BPD/81830/2011.pt_PT
dc.identifier10.3390/ijms151017541
dc.identifier.citationCruz, T.M.D.; Carvalho, R.F.; Richardson, D.N.; Duque, P. Abscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expression. Int. J. Mol. Sci. 2014, 15, 17541-17564.pt_PT
dc.identifier.doi10.3390/ijms151017541
dc.identifier.doi10.3390/ijms151017541
dc.identifier.urihttp://hdl.handle.net/10400.7/335
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMolecular characterization of a novel RNA-binding protein conferring salt and drought tolerance during seed germination
dc.relation.publisherversionhttp://www.mdpi.com/1422-0067/15/10/17541pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectabscisic acid (ABA)pt_PT
dc.subjectABA-responsive cis elementspt_PT
dc.subjectalternative splicing (AS)pt_PT
dc.subjectArabidopsis thalianapt_PT
dc.subjectgene expressionpt_PT
dc.subjectSR proteinspt_PT
dc.titleAbscisic Acid (ABA) Regulation of Arabidopsis SR Protein Gene Expressionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular characterization of a novel RNA-binding protein conferring salt and drought tolerance during seed germination
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-PRO%2F119058%2F2010/PT
oaire.citation.endPage17564pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage17541pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume15pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication53012bcc-50c1-4e9f-a7c7-849eff26ee14
relation.isProjectOfPublication.latestForDiscovery53012bcc-50c1-4e9f-a7c7-849eff26ee14

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