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Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone

2011-07-31Journal article Open access
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dc.contributor.authorvan de Ven, C.
dc.contributor.authorBialecka, M.
dc.contributor.authorNeijts, R.
dc.contributor.authorYoung, T.
dc.contributor.authorRowland, J. E.
dc.contributor.authorStringer, E. J.
dc.contributor.authorVan Rooijen, C.
dc.contributor.authorMeijlink, F.
dc.contributor.authorNovoa, A.
dc.contributor.authorFreund, J.-N.
dc.contributor.authorMallo, M.
dc.contributor.authorBeck, F.
dc.contributor.authorDeschamps, J.
dc.date.accessioned2016-06-14T14:45:28Z
dc.date.available2016-06-14T14:45:28Z
dc.date.issued2011-07-31
dc.description.abstractDecrease in Cdx dosage in an allelic series of mouse Cdx mutants leads to progressively more severe posterior vertebral defects. These defects are corrected by posterior gain of function of the Wnt effector Lef1. Precocious expression of Hox paralogous 13 genes also induces vertebral axis truncation by antagonizing Cdx function. We report here that the phenotypic similarity also applies to patterning of the caudal neural tube and uro-rectal tracts in Cdx and Wnt3a mutants, and in embryos precociously expressing Hox13 genes. Cdx2 inactivation after placentation leads to posterior defects, including incomplete uro-rectal septation. Compound mutants carrying one active Cdx2 allele in the Cdx4-null background (Cdx2/4), transgenic embryos precociously expressing Hox13 genes and a novel Wnt3a hypomorph mutant all manifest a comparable phenotype with similar uro-rectal defects. Phenotype and transcriptome analysis in early Cdx mutants, genetic rescue experiments and gene expression studies lead us to propose that Cdx transcription factors act via Wnt signaling during the laying down of uro-rectal mesoderm, and that they are operative in an early phase of these events, at the site of tissue progenitors in the posterior growth zone of the embryo. Cdx and Wnt mutations and premature Hox13 expression also cause similar neural dysmorphology, including ectopic neural structures that sometimes lead to neural tube splitting at caudal axial levels. These findings involve the Cdx genes, canonical Wnt signaling and the temporal control of posterior Hox gene expression in posterior morphogenesis in the different embryonic germ layers. They shed a new light on the etiology of the caudal dysplasia or caudal regression range of human congenital defects.pt_PT
dc.description.sponsorshipAICR project grant: (08-0199); Dutch Earth and Life Sciences grant: (820.02.005); 6th Framework Programme Network of Excellence `Cells into Organs'; Dutch government grant: (Bsik Program 03038); Fundação para a Ciência e Tecnologia grant: (PTDC/BIA-BCM/110638/2009); Centro de Biologia do Desenvolvimento grant: (POCTI-ISFL-4-664).pt_PT
dc.identifier.citationConcerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone Cesca van de Ven, Monika Bialecka, Roel Neijts, Teddy Young, Jennifer E. Rowland, Emma J. Stringer, Carina Van Rooijen, Frits Meijlink, Ana Nóvoa, Jean-Noel Freund, Moises Mallo, Felix Beck, Jacqueline Deschamps Development 2011 138: 3451-3462; doi: 10.1242/dev.066118pt_PT
dc.identifier.doi10.1242/dev.066118pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/644
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCompany of Biologistspt_PT
dc.relation.publisherversionhttp://dev.biologists.org/content/138/16/3451pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectCell Shapept_PT
dc.subjectEmbryo, Mammalianpt_PT
dc.subjectFemalept_PT
dc.subjectHedgehog Proteinspt_PT
dc.subjectHomeodomain Proteinspt_PT
dc.subjectMalept_PT
dc.subjectMicept_PT
dc.subjectMice, Inbred C57BLpt_PT
dc.subjectMice, Transgenicpt_PT
dc.subjectNeural Tubept_PT
dc.subjectTranscription Factorspt_PT
dc.subjectTretinoinpt_PT
dc.subjectWnt Proteinspt_PT
dc.subjectWnt3 Proteinpt_PT
dc.subjectWnt3A Proteinpt_PT
dc.subjectGene Expression Regulation, Developmentalpt_PT
dc.subjectSignal Transductionpt_PT
dc.titleConcerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zonept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3462pt_PT
oaire.citation.issue16pt_PT
oaire.citation.startPage3451pt_PT
oaire.citation.titleDevelopmentpt_PT
oaire.citation.volume138pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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