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Regulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT)

2012-07-31Journal article Open access
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dc.contributor.authorPejanovic, Nadja
dc.contributor.authorHochrainer, Karin
dc.contributor.authorLiu, Tao
dc.contributor.authorAerne, Birgit L.
dc.contributor.authorSoares, Miguel P.
dc.contributor.authorAnrather, Josef
dc.date.accessioned2016-06-23T10:04:44Z
dc.date.available2016-06-23T10:04:44Z
dc.date.issued2012-07-31
dc.description.abstractThe NF-κB family member p65 is central to inflammation and immunity. The purpose of this study was to identify and characterize evolutionary conserved genes modulating p65 transcriptional activity. Using an RNAi screening approach, we identified chaperonin containing TCP1 subunit η (CCTη) as a regulator of Drosophila NF-κB proteins, Dorsal and Dorsal-related immunity factor (Dif). CCTη was also found to regulate NF-κB-driven transcription in mammalian cells, acting in a promoter-specific context, downstream of IκB kinase (IKK). CCTη knockdown repressed IκBα and CXCL2/MIP2 transcription during the early phase of NF-κB activation while impairing the termination of CCL5/RANTES and CXCL10/IP10 transcription. The latter effect was associated with increased DNA binding and reduced p65 acetylation, presumably by altering the activity of histone acetyltransferase CREB-binding protein (CBP). We identified p65 lysines (K) 122 and 123 as target residues mediating the CCTη-driven termination of NF-κB-dependent transcription. We propose that CCTη regulates NF-κB activity in a manner that resolves inflammation.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia grants: (SFRH/BD/28016/2006, PTDC/BIA-BCM/101311/2008, PTDC/SAU-FCF/100762/2008); European Community grant: (LSH-2005-1.2.5-); National Institutes of Health grant: (HL077308).pt_PT
dc.identifier.citationPejanovic N, Hochrainer K, Liu T, Aerne BL, Soares MP, et al. (2012) Regulation of Nuclear Factor k B(NF- k B) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT). PLoS ONE 7(7): e42020. doi:10.1371/journal.pone.0042020pt_PT
dc.identifier.doi10.1371/journal.pone.0042020pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/661
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relation100762pt_PT
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042020pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAcetylationpt_PT
dc.subjectAnimalspt_PT
dc.subjectBase Sequencept_PT
dc.subjectBlotting, Westernpt_PT
dc.subjectCells, Culturedpt_PT
dc.subjectChaperoninspt_PT
dc.subjectDNA Primerspt_PT
dc.subjectDrosophilapt_PT
dc.subjectElectrophoretic Mobility Shift Assaypt_PT
dc.subjectGene Knockdown Techniquespt_PT
dc.subjectNF-kappa Bpt_PT
dc.subjectPolymerase Chain Reactionpt_PT
dc.subjectRNA Interferencept_PT
dc.subjectTranscription, Geneticpt_PT
dc.titleRegulation of Nuclear Factor κB (NF-κB) Transcriptional Activity via p65 Acetylation by the Chaperonin Containing TCP1 (CCT)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage10pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume7pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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