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Rab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate

2015-05-11Journal article Open access
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dc.contributor.authorGerber, P. P.
dc.contributor.authorCabrini, M.
dc.contributor.authorJancic, C.
dc.contributor.authorPaoletti, L.
dc.contributor.authorBanchio, C.
dc.contributor.authorvon Bilderling, C.
dc.contributor.authorSigaut, L.
dc.contributor.authorPietrasanta, L. I.
dc.contributor.authorDuette, G.
dc.contributor.authorFreed, E. O.
dc.contributor.authorde Saint Basile, G.
dc.contributor.authorMoita, C. F.
dc.contributor.authorMoita, L. F.
dc.contributor.authorAmigorena, S.
dc.contributor.authorBenaroch, P.
dc.contributor.authorGeffner, J.
dc.contributor.authorOstrowski, M.
dc.date.accessioned2015-10-20T14:12:27Z
dc.date.available2015-10-20T14:12:27Z
dc.date.issued2015-05-11
dc.description.abstractDuring the late stages of the HIV-1 replication cycle, the viral polyprotein Pr55(Gag) is recruited to the plasma membrane (PM), where it binds phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and directs HIV-1 assembly. We show that Rab27a controls the trafficking of late endosomes carrying phosphatidylinositol 4-kinase type 2 α (PI4KIIα) toward the PM of CD4(+) T cells. Hence, Rab27a promotes high levels of PM phosphatidylinositol 4-phosphate and the localized production of PI(4,5)P2, therefore controlling Pr55(Gag) membrane association. Rab27a also controls PI(4,5)P2 levels at the virus-containing compartments of macrophages. By screening Rab27a effectors, we identified that Slp2a, Slp3, and Slac2b are required for the association of Pr55(Gag) with the PM and that Slp2a cooperates with Rab27a in the recruitment of PI4KIIα to the PM. We conclude that by directing the trafficking of PI4KIIα-positive endosomes toward the PM, Rab27a controls PI(4,5)P2 production and, consequently, HIV-1 replication.pt_PT
dc.description.sponsorshipUniversidad de Buenos Aires and CONICET doctoral fellowships, Agencia Nacional de Pro- moción Científica y Tecnológica (Argentina) grants: (2010-1681, 2012-00353), Creative and Novel Ideas in HIV Research Program, University of Alabama at Birmingham Center for AIDS Research funding grant P30 AI027767-24.pt_PT
dc.identifier10.1083/jcb.201409082
dc.identifier.citationRab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphate Pehuén Pereyra Gerber, Mercedes Cabrini, Carolina Jancic, Luciana Paoletti, Claudia Banchio, Catalina von Bilderling, Lorena Sigaut, Lía I. Pietrasanta, Gabriel Duette, Eric O. Freed, Genevieve de Saint Basile, Catarina Ferreira Moita, Luis Ferreira Moita, Sebastian Amigorena, Philippe Benaroch, Jorge Geffner, and Matías Ostrowski J Cell Biol 2015 209:435-452. Published May 4, 2015, doi:10.1083/jcb.201409082pt_PT
dc.identifier.doi10.1083/jcb.201409082
dc.identifier.urihttp://hdl.handle.net/10400.7/411
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherThe Rockefeller University Presspt_PT
dc.relation.publisherversionhttp://jcb.rupress.org/content/209/3/435.long#abstract-2pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHIV-1pt_PT
dc.titleRab27a controls HIV-1 assembly by regulating plasma membrane levels of phosphatidylinositol 4,5-bisphosphatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage452pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage435pt_PT
oaire.citation.titleThe Journal of Cell Biologypt_PT
oaire.citation.volume209pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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