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Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells

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dc.contributor.authorAlvarez, Catalina
dc.contributor.authorCampos, Ana Claudia
dc.contributor.authorHoward, Jonathan C
dc.contributor.authorLoureiro, Joana
dc.contributor.authorMüller, Urs Benedikt
dc.contributor.authorRodrigues, Ana Lina
dc.date.accessioned2020-03-11T10:37:18Z
dc.date.available2020-03-11T10:37:18Z
dc.date.issued2020
dc.description.abstractCritical steps in resistance of mice against Toxoplasma gondii occur in the first 2 or 3 h after the pathogen has entered a cell that has been exposed to interferon γ (IFNγ). The newly formed parasitophorous vacuole is attacked by the IFNγ-inducible IRG proteins and disrupted, resulting in death of the parasite and necrotic death of the cell. Here we describe some techniques that we have used to describe and quantify these events in different combinations of the host and the parasite.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlvarez C, Campos AC, Howard JC, Loureiro J, Müller UB, Rodrigues AL. Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells. Methods Mol Biol. 2020;2071:371–409pt_PT
dc.identifier.doi10.1007/978-1-4939-9857-9_20pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/929
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationCytoplasmic sensing of Toxoplasma gondii infection and host cell programmed necrosis
dc.subjectResearch Subject Categories::NATURAL SCIENCESpt_PT
dc.subjectToxoplasma gondii Parasitophorous vacuole Mouse Innate immunity IRG proteins Interferon-γ Diaphragm-derived cells Reactive necrosis Flow cytometry Immunofluorescencept_PT
dc.titleMethods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cellspt_PT
dc.typebook part
dspace.entity.typePublication
oaire.awardTitleCytoplasmic sensing of Toxoplasma gondii infection and host cell programmed necrosis
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/708694/EU
oaire.citation.endPage409pt_PT
oaire.citation.startPage371pt_PT
oaire.citation.titleMethods in molecular biology (Clifton, N.J.)pt_PT
oaire.citation.volume2071pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typebookPartpt_PT
relation.isProjectOfPublication876063fa-6f8e-4190-8469-75386e0a23f7
relation.isProjectOfPublication.latestForDiscovery876063fa-6f8e-4190-8469-75386e0a23f7

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