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Transient Activation of Meox1 Is an Early Component of the Gene Regulatory Network Downstream of Hoxa2

2011-03Journal article Open access
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dc.contributor.authorKirilenko, P.
dc.contributor.authorHe, G.
dc.contributor.authorMankoo, B. S.
dc.contributor.authorMallo, M.
dc.contributor.authorJones, R.
dc.contributor.authorBobola, N.
dc.date.accessioned2016-06-29T09:23:05Z
dc.date.available2016-06-29T09:23:05Z
dc.date.issued2011-03
dc.description.abstractHox genes encode transcription factors that regulate morphogenesis in all animals with bilateral symmetry. Although Hox genes have been extensively studied, their molecular function is not clear in vertebrates, and only a limited number of genes regulated by Hox transcription factors have been identified. Hoxa2 is required for correct development of the second branchial arch, its major domain of expression. We now show that Meox1 is genetically downstream from Hoxa2 and is a direct target. Meox1 expression is downregulated in the second arch of Hoxa2 mouse mutant embryos. In chromatin immunoprecipitation (ChIP), Hoxa2 binds to the Meox1 proximal promoter. Two highly conserved binding sites contained in this sequence are required for Hoxa2-dependent activation of the Meox1 promoter. Remarkably, in the absence of Meox1 and its close homolog Meox2, the second branchial arch develops abnormally and two of the three skeletal elements patterned by Hoxa2 are malformed. Finally, we show that Meox1 can specifically bind the DNA sequences recognized by Hoxa2 on its functional target genes. These results provide new insight into the Hoxa2 regulatory network that controls branchial arch identity.pt_PT
dc.description.sponsorshipBBSRC grant: (BB/E017355/1); Manchester Academic Health Science Centre; Manchester NIHR Biomedical Research Centre; FCT grant: (PTDC/BIA-BCM/71619/2006); Centro de Biologia do Desenvolvimento grant: (POCTI-ISFL-4-664).pt_PT
dc.identifier.citationTransient Activation of Meox1 Is an Early Component of the Gene Regulatory Network Downstream of Hoxa2 Pavel Kirilenko, Guiyuan He, Baljinder S. Mankoo, Moises Mallo, Richard Jones, and Nicoletta Bobola Mol. Cell. Biol. March 15, 2011 31:6 1301-1308; Accepted manuscript posted online 18 January 2011, doi:10.1128/MCB.00705-10pt_PT
dc.identifier.doi10.1128/MCB.00705-10pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/670
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttp://mcb.asm.org/content/31/6/1301.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBase Sequencept_PT
dc.subjectBranchial Regionpt_PT
dc.subjectCells, Culturedpt_PT
dc.subjectEmbryo, Mammalianpt_PT
dc.subjectHomeodomain Proteinspt_PT
dc.subjectHumanspt_PT
dc.subjectMicept_PT
dc.subjectMice, Transgenicpt_PT
dc.subjectMolecular Sequence Datapt_PT
dc.subjectMutationpt_PT
dc.subjectPromoter Regions, Geneticpt_PT
dc.subjectProtein Bindingpt_PT
dc.subjectSequence Alignmentpt_PT
dc.subjectGene Expression Regulation, Developmentalpt_PT
dc.subjectGene Regulatory Networkspt_PT
dc.titleTransient Activation of Meox1 Is an Early Component of the Gene Regulatory Network Downstream of Hoxa2pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1308pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1301pt_PT
oaire.citation.titleMolecular and Cellular Biologypt_PT
oaire.citation.volume31pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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