Repository logo
 
Publication

A Genomic Signature and the Identification of New Sporulation Genes

2013Journal article Open access
Simple item page
dc.contributor.authorAbecasis, A. B.
dc.contributor.authorSerrano, M.
dc.contributor.authorAlves, R.
dc.contributor.authorQuintais, L.
dc.contributor.authorPereira-Leal, J. B.
dc.contributor.authorHenriques, A. O.
dc.date.accessioned2015-10-21T12:12:58Z
dc.date.available2015-10-21T12:12:58Z
dc.date.issued2013
dc.description.abstractBacterial endospores are the most resistant cell type known to humans, as they are able to withstand extremes of temperature, pressure, chemical injury, and time. They are also of interest because the endospore is the infective particle in a variety of human and livestock diseases. Endosporulation is characterized by the morphogenesis of an endospore within a mother cell. Based on the genes known to be involved in endosporulation in the model organism Bacillus subtilis, a conserved core of about 100 genes was derived, representing the minimal machinery for endosporulation. The core was used to define a genomic signature of about 50 genes that are able to distinguish endospore-forming organisms, based on complete genome sequences, and we show this 50-gene signature is robust against phylogenetic proximity and other artifacts. This signature includes previously uncharacterized genes that we can now show are important for sporulation in B. subtilis and/or are under developmental control, thus further validating this genomic signature. We also predict that a series of polyextremophylic organisms, as well as several gut bacteria, are able to form endospores, and we identified 3 new loci essential for sporulation in B. subtilis: ytaF, ylmC, and ylzA. In all, the results support the view that endosporulation likely evolved once, at the base of the Firmicutes phylum, and is unrelated to other bacterial cell differentiation programs and that this involved the evolution of new genes and functions, as well as the cooption of ancestral, housekeeping functions.pt_PT
dc.description.sponsorshipFCT grant: (PEst-OE/EQB/LA0004/2011), FCT Ph.D. fellowship: (SFRH/BPD/36328/2007), FCT postdoc fellowship: (SFRH/BPD/65605/2009), Instituto Gulbenkian de Ciência research fellowship.pt_PT
dc.identifier10.1128/JB.02110-12
dc.identifier.citationA Genomic Signature and the Identification of New Sporulation Genes Ana B. Abecasis, Mónica Serrano, Renato Alves, Leonor Quintais, José B. Pereira-Leal, and Adriano O. Henriques J. Bacteriol. May 2013 195:9 2101-2115; Accepted manuscript posted online 8 February 2013, doi:10.1128/JB.02110-12pt_PT
dc.identifier.doi10.1128/JB.02110-12
dc.identifier.urihttp://hdl.handle.net/10400.7/421
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationPerda de robustez como um princípio de reposicionamento de fármacos
dc.relation.publisherversionhttp://jb.asm.org/content/195/9/2101.longpt_PT
dc.subjectGenespt_PT
dc.subjectGenomicspt_PT
dc.titleA Genomic Signature and the Identification of New Sporulation Genespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePerda de robustez como um princípio de reposicionamento de fármacos
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/ERA-PTG%2FSAU%2F0002%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-BIO%2F119006%2F2010/PT
oaire.citation.endPage2115pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage2101pt_PT
oaire.citation.titleJournal of Bacteriologypt_PT
oaire.citation.volume195pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationa8455abd-7c17-434e-98c6-3326c9501a28
relation.isProjectOfPublication38d12f4c-99a0-4d41-a564-c1a2b7f52311
relation.isProjectOfPublication.latestForDiscoverya8455abd-7c17-434e-98c6-3326c9501a28

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
J. Bacteriol.-2013-Abecasis-2101-15.pdf
Size:
2.47 MB
Format:
Adobe Portable Document Format
Description:
artigo principal
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CG - Artigos