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KIF13A mediates influenza a virus ribonucleoproteins trafficking

2017-10-23Journal article Open access
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dc.contributor.authorRamos-Nascimento, Ana
dc.contributor.authorKellen, Bárbara
dc.contributor.authorFerreira, Filipe
dc.contributor.authorAlenquer, Marta
dc.contributor.authorVale-Costa, Silvia
dc.contributor.authorRaposo, Graça
dc.contributor.authorDelevoye, Cédric
dc.contributor.authorAmorim, Maria João
dc.date.accessioned2017-10-30T17:34:27Z
dc.date.available2018-11-01T01:30:09Z
dc.date.issued2017-10-23
dc.descriptionThe deposited article version contains attached the supplementary materials within the pdf. This publication hasn't any creative commons license associated. The deposited article version is a "JCS Advance Online Article" provided by The Company of Biologists, and it is the "Accepted manuscript" posted online on 23 October 2017.pt_PT
dc.description.abstractInfluenza A is a rapid evolving virus, successful in provoking periodic epidemics and occasional pandemics in humans. Viral assembly is complex as the virus incorporates an eight-partite segmented genome of RNA (in the form of viral ribonucleoproteins, vRNPs). Genome assembly, with implications to public health, is not completely understood. It was reported that vRNPs are transported to the cell surface on Rab11 vesicles using microtubules, but no molecular motor has been assigned to the process. Here, we have identified KIF13A, a member of the kinesin-3 family, as the first molecular motor efficiently transporting vRNP-Rab11 vesicles during IAV infection. Depletion of KIF13A resulted in reduced viral titres and less accumulation of vRNPs at the cell surface, without interfering with the levels of other viral proteins at sites of viral assembly. In addition, in overexpression conditions and using two artificial methods able to displace vRNP-Rab11 vesicles, KIF13A augmented vRNP levels at the plasma membrane. Together our results show that KIF13A is an important host factor promoting influenza A vRNP transport, which is a crucial step for viral assembly.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grants: (PTDC/IMI-MIC/1142/2012, IF/00899/2013, SFRH/BPD/62982/2009, SFRH/BPD/94204/2013); Instituto Calouste Gulbenkian; Fundação Calouste Gulbenkian; Fondation pour la Recherche Médicale grant: (Equipe FRM DEQ20140329491 Team label); Fondation ARC pour la Recherche sur le Cancer grant: (PJA20161204965); CNRS; INSERM; Institut Curie.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.doi10.1242/jcs.210807pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/795
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCompany of Biologistspt_PT
dc.relationIF/00899/2013pt_PT
dc.relationROLE OF MITOFUSIN 2 DURING INFLUENZA A VIRUS INFECTION
dc.relationMolecular characterization of the cellular machinery involved in genome trafficking of influenza A virus
dc.relation.publisherversionhttp://jcs.biologists.org/content/early/2017/10/19/jcs.210807pt_PT
dc.subjectKIF13Apt_PT
dc.subjectRecycling endosomept_PT
dc.subjectInfluenza A virus assemblypt_PT
dc.subjectMolecular motorpt_PT
dc.titleKIF13A mediates influenza a virus ribonucleoproteins traffickingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleROLE OF MITOFUSIN 2 DURING INFLUENZA A VIRUS INFECTION
oaire.awardTitleMolecular characterization of the cellular machinery involved in genome trafficking of influenza A virus
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FIMI-MIC%2F1142%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F62982%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F94204%2F2013/PT
oaire.citation.endPage48pt_PT
oaire.citation.issueAccepted manuscriptpt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Cell Sciencept_PT
oaire.citation.volumeAccepted manuscriptpt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctThe deposited article has 13 months of embargo period in terms of access, because of the journal's policies and the publisher's copyright policy. Since the paper has not been published yet, the library service put in this upload an embargo period superior than 12 months, as a securing measure.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb3129c8c-ee05-4456-a36c-13de51208da7
relation.isProjectOfPublicationbde69ba6-c27b-4af5-a3d6-6517d8b2236f
relation.isProjectOfPublicationa6608970-1624-4621-a0df-f1c0cd22766f
relation.isProjectOfPublication.latestForDiscoverybde69ba6-c27b-4af5-a3d6-6517d8b2236f

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