Microbial Diversity and Toxin Risk in Tropical Freshwater Reservoirs of Cape Verde

Semedo-Aguiar, Ana; Pereira-Leal, Jose; Leite, Ricardo

http://hdl.handle.net/10400.7/866

Utilize este identificador para referenciar este registo.

Nome:	Descrição:	Tamanho:	Formato:
Leite,R._Toxins_(2018).pdf	main article	6.74 MB	Adobe PDF	Ver/Abrir
Leite,R._Toxins_(2018)_SM_.pdf	supplementary materials	458.91 KB	Adobe PDF	Ver/Abrir

Contacte-nos

Autores

Semedo-Aguiar, Ana

Pereira-Leal, Jose

Leite, Ricardo

Resumo(s)

The Cape Verde islands are part of the African Sahelian arid belt that possesses an erratic rain pattern prompting the need for water reservoirs, which are now critical for the country’s sustainability. Worldwide, freshwater cyanobacterial blooms are increasing in frequency due to global climate change and the eutrophication of water bodies, particularly in reservoirs. To date, there have been no risk assessments of cyanobacterial toxin production in these man-made structures. We evaluated this potential risk using 16S rRNA gene amplicon sequencing and full metagenome sequencing in freshwater reservoirs of Cape Verde. Our analysis revealed the presence of several potentially toxic cyanobacterial genera in all sampled reservoirs. Faveta potentially toxic and bloom-forming Microcystis sp., dominated our samples, while a Cryptomonas green algae and Gammaproteobacteria dominated Saquinho and Poilão reservoirs. We reconstructed and assembled the Microcystis genome, extracted from the metagenome of bulk DNA from Faveta water. Phylogenetic analysis of Microcystis cf. aeruginosa CV01’s genome revealed its close relationship with other Microcystis genomes, as well as clustering with other continental African strains, suggesting geographical coherency. In addition, it revealed several clusters of known toxin-producing genes. This survey reinforces the need to better understand the country’s microbial ecology as a whole of water reservoirs on the rise.

Descrição

This deposit is composed by the main article plus the supplementary materials of the publication.

Palavras-chave

toxins Cyanobacteria NGS Microcystis MAGs metagenome freshwater microbial communities Cape Verde reservoirs

URI

http://hdl.handle.net/10400.7/866

Citação

Semedo-Aguiar, A.P.; Pereira-Leal, J.B.; Leite, R.B. Microbial Diversity and Toxin Risk in Tropical Freshwater Reservoirs of Cape Verde. Toxins 2018, 10, 186.