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Increased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages

2013-01Journal article Open access
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dc.contributor.authorMiskinyte, M.
dc.contributor.authorGordo, I.
dc.date.accessioned2015-11-03T12:56:30Z
dc.date.available2015-11-03T12:56:30Z
dc.date.issued2013-01
dc.description.abstractMutations causing antibiotic resistance usually incur a fitness cost in the absence of antibiotics. The magnitude of such costs is known to vary with the environment. Little is known about the fitness effects of antibiotic resistance mutations when bacteria confront the host's immune system. Here, we study the fitness effects of mutations in the rpoB, rpsL, and gyrA genes, which confer resistance to rifampin, streptomycin, and nalidixic acid, respectively. These antibiotics are frequently used in the treatment of bacterial infections. We measured two important fitness traits-growth rate and survival ability-of 12 Escherichia coli K-12 strains, each carrying a single resistance mutation, in the presence of macrophages. Strikingly, we found that 67% of the mutants survived better than the susceptible bacteria in the intracellular niche of the phagocytic cells. In particular, all E. coli streptomycin-resistant mutants exhibited an intracellular advantage. On the other hand, 42% of the mutants incurred a high fitness cost when the bacteria were allowed to divide outside of macrophages. This study shows that single nonsynonymous changes affecting fundamental processes in the cell can contribute to prolonged survival of E. coli in the context of an infection.pt_PT
dc.description.sponsorshipEuropean Research Council, LAO/ITQB, FCT.pt_PT
dc.identifier10.1128/AAC.01632-12
dc.identifier.citationIncreased Survival of Antibiotic-Resistant Escherichia coli inside Macrophages Migla Miskinyte and Isabel Gordo Antimicrob. Agents Chemother. January 2013 57:1 189-195; Accepted manuscript posted online 22 October 2012, doi:10.1128/AAC.01632-12pt_PT
dc.identifier.doi10.1128/AAC.01632-12
dc.identifier.urihttp://hdl.handle.net/10400.7/463
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationMicrobial adaptation within ecosystems
dc.relation.publisherversionhttp://aac.asm.org/content/57/1/189.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectEscherichia coli K12pt_PT
dc.subjectEscherichia coli Proteinspt_PT
dc.subjectMacrophagespt_PT
dc.titleIncreased Survival of Antibiotic-Resistant Escherichia coli inside Macrophagespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.citation.endPage195pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage189pt_PT
oaire.citation.titleAntimicrobial Agents and Chemotherapypt_PT
oaire.citation.volume57pt_PT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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