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miRNAs mediate SnRK1-dependent energy signaling in Arabidopsis

2013-06-20Journal article Open access
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dc.contributor.authorConfraria, Ana
dc.contributor.authorMartinho, Cláudia
dc.contributor.authorElias, Alexandre
dc.contributor.authorRubio-Somoza, Ignacio
dc.contributor.authorBaena-González, Elena
dc.date.accessioned2015-10-26T13:02:06Z
dc.date.available2015-10-26T13:02:06Z
dc.date.issued2013-06-20
dc.description.abstractThe SnRK1 protein kinase, the plant ortholog of mammalian AMPK and yeast Snf1, is activated by the energy depletion caused by adverse environmental conditions. Upon activation, SnRK1 triggers extensive transcriptional changes to restore homeostasis and promote stress tolerance and survival partly through the inhibition of anabolism and the activation of catabolism. Despite the identification of a few bZIP transcription factors as downstream effectors, the mechanisms underlying gene regulation, and in particular gene repression by SnRK1, remain mostly unknown. microRNAs (miRNAs) are 20-24 nt RNAs that regulate gene expression post-transcriptionally by driving the cleavage and/or translation attenuation of complementary mRNA targets. In addition to their role in plant development, mounting evidence implicates miRNAs in the response to environmental stress. Given the involvement of miRNAs in stress responses and the fact that some of the SnRK1-regulated genes are miRNA targets, we postulated that miRNAs drive part of the transcriptional reprogramming triggered by SnRK1. By comparing the transcriptional response to energy deprivation between WT and dcl1-9, a mutant deficient in miRNA biogenesis, we identified 831 starvation genes misregulated in the dcl1-9 mutant, out of which 155 are validated or predicted miRNA targets. Functional clustering analysis revealed that the main cellular processes potentially co-regulated by SnRK1 and miRNAs are translation and organelle function and uncover TCP transcription factors as one of the most highly enriched functional clusters. TCP repression during energy deprivation was impaired in miR319 knockdown (MIM319) plants, demonstrating the involvement of miR319 in the stress-dependent regulation of TCPs. Altogether, our data indicates that miRNAs are components of the SnRK1 signaling cascade contributing to the regulation of specific mRNA targets and possibly tuning down particular cellular processes during the stress response.pt_PT
dc.description.sponsorshipFCT fellowships: (SFRH/BPD/47280/2008, SFRH/BD/33563/2008), EMBOLong-Term Fellowship, Gottfried Wilhelm Leibniz Award, Max Planck Society funds, Marie Curie IRG grant, EMBO Installation grant (FCT), Marie Curie Actions FP7-People-2010-ITN.pt_PT
dc.identifier10.3389/fpls.2013.00197
dc.identifier.citationConfrariaA,MartinhoC, EliasA,Rubio-SomozaIandBaena- GonzálezE(2013) miRNAsmediate SnRK1-dependentenergysignalingin Arabidopsis.Front.PlantSci. 4:197.doi: 10.3389/fpls.2013.00197pt_PT
dc.identifier.doi10.3389/fpls.2013.00197
dc.identifier.urihttp://hdl.handle.net/10400.7/442
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Research Foundationpt_PT
dc.relation.publisherversionhttp://journal.frontiersin.org/article/10.3389/fpls.2013.00197/abstractpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectArabidopsispt_PT
dc.subjectSnRK1pt_PT
dc.subjectstresspt_PT
dc.subjectmiRNApt_PT
dc.subjectenergy signalingpt_PT
dc.subjectDCL1pt_PT
dc.titlemiRNAs mediate SnRK1-dependent energy signaling in Arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F107924%2F2008/PT
oaire.citation.endPage21pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFrontiers in Plant Sciencept_PT
oaire.citation.volume4pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication436889e6-e37e-40a8-a5af-d42e0f1b97e3
relation.isProjectOfPublication.latestForDiscovery436889e6-e37e-40a8-a5af-d42e0f1b97e3

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