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Evolution of Escherichia coli to Macrophage Cell Line

2014-09Journal article Open access
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dc.contributor.authorMiskinyte, Migla
dc.contributor.authorGordo, Isabel
dc.date.accessioned2015-12-21T19:08:06Z
dc.date.available2015-12-21T19:08:06Z
dc.date.issued2014-09
dc.description.abstractThe genomes of species of Escherichia coli (E. coli) show an extraordinary amount of diversity, which include commensal strains and strains belonging to different pathovars. Many strains of E. coli, which can cause mild or severe pathologies in humans, have a commensal ancestor. Understanding the evolutionary changes that can lead to a transition from commensal to pathogen is an important task, which requires integration of different methodologies. One method is experimental evolution of bacteria, in controlled environments, that mimic some of the selective pressures, likely to be important during the transition to pathogenesis. The success of such a transition will depend, at least partially, on ability of E. coli to adapt to the presence of cells of the immune system. Here, we describe a protocol for performing experimental evolution of a commensal strain of E. coli, a derivative of the well studied K12, under the constant selective pressure imposed by cells of the innate immune system, specifically RAW 264.7 murine macrophage cell line.pt_PT
dc.description.sponsorshipLAO/ITQB, FCT.pt_PT
dc.identifier.citationMiskinyte, M. and Gordo, I. (2014). Evolution of Escherichia coli to Macrophage Cell Line. Bio-protocol 4(17): e1227. http://www.bio-protocol.org/e1227pt_PT
dc.identifier.otherhttp://www.bio-protocol.org/e1227
dc.identifier.urihttp://hdl.handle.net/10400.7/541
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBio-Protocolpt_PT
dc.relationMicrobial adaptation within ecosystems
dc.relation.publisherversionhttp://www.bio-protocol.org/e1227pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMicrobiologypt_PT
dc.subjectMicrobe-host interactionspt_PT
dc.subjectBacteriumpt_PT
dc.subjectMicrobial geneticspt_PT
dc.subjectDNApt_PT
dc.subjectDNA replicationpt_PT
dc.subjectMolecular Biologypt_PT
dc.subjectGenotypingpt_PT
dc.subjectBacteriapt_PT
dc.subjectEscherichiapt_PT
dc.subjectEscherichia colipt_PT
dc.titleEvolution of Escherichia coli to Macrophage Cell Linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.citation.endPage6pt_PT
oaire.citation.issue17pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlebio-protocolpt_PT
oaire.citation.volume4pt_PT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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