Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

Vale-Costa, Sílvia; Alenquer, Marta; Sousa, Ana Laura; Kellen, Bárbara; Ramalho, José; Tranfield, Erin M.; Amorim, Maria João

Publication

Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors

2016-04-15Journal article

dc.contributor.author	Vale-Costa, Sílvia
dc.contributor.author	Alenquer, Marta
dc.contributor.author	Sousa, Ana Laura
dc.contributor.author	Kellen, Bárbara
dc.contributor.author	Ramalho, José
dc.contributor.author	Tranfield, Erin M.
dc.contributor.author	Amorim, Maria João
dc.date.accessioned	2016-06-08T11:38:59Z
dc.date.issued	2016-04-15
dc.description.abstract	Influenza A virus assembly is an unclear process, whereby individual virion components form an infectious particle. The segmented nature of the influenza A genome imposes a problem to assembly because it requires packaging of eight distinct RNA particles (vRNPs). It also allows genome mixing from distinct parental strains, events associated with influenza pandemic outbreaks. It is important to public health to understand how segmented genomes assemble, a process that is dependent on the transport of components to assembly sites. Previously, it has been shown that vRNPs are carried by recycling endosome vesicles, resulting in a change of Rab11 distribution. Here, we describe that vRNP binding to recycling endosomes impairs recycling endosome function, by competing for Rab11 binding with family-interacting proteins, and that there is a causal relationship between Rab11 ability to recruit family-interacting proteins and Rab11 redistribution. This competition reduces recycling sorting at an unclear step, resulting in clustering of single- and double-membraned vesicles. These morphological changes in Rab11 membranes are indicative of alterations in protein and lipid homeostasis during infection. Vesicular clustering creates hotspots of the vRNPs that need to interact to form an infectious particle.	pt_PT
dc.description.sponsorship	Fundação para a Ciência e Tecnologia grants: (PTDC/IMI-MIC/1142/2012, SFRH/BPD/94204/2013, SFRH/BPD/62982/2009, IF/00899/2013); Fundação Calouste Gulbenkian; Instituto Gulbenkian de Ciência.	pt_PT
dc.identifier.citation	Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors Sílvia Vale-Costa, Marta Alenquer, Ana Laura Sousa, Bárbara Kellen, José Ramalho, Erin M. Tranfield, Maria João Amorim J Cell Sci 2016 129: 1697-1710; doi: 10.1242/jcs.188409	pt_PT
dc.identifier.doi	10.1242/jcs.188409	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/628
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Company of Biologists	pt_PT
dc.relation.publisherversion	http://jcs.biologists.org/content/129/8/1697.long	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Correlative light and electron microscopy	pt_PT
dc.subject	Rab11	pt_PT
dc.subject	Influenza A virus assembly	pt_PT
dc.subject	Membrane trafficking	pt_PT
dc.subject	Recycling endosome	pt_PT
dc.title	Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	1710	pt_PT
oaire.citation.issue	8	pt_PT
oaire.citation.startPage	1697	pt_PT
oaire.citation.title	Journal of Cell Science	pt_PT
oaire.citation.volume	129	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT