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Robust gap repair in the contractile ring ensures timely completion of cytokinesis

2016-12-19Journal article Open access
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dc.contributor.authorSilva, Ana M
dc.contributor.authorOsório, Daniel S
dc.contributor.authorPereira, Antonio J
dc.contributor.authorMaiato, Helder
dc.contributor.authorPinto, Inês Mendes
dc.contributor.authorRubinstein, Boris
dc.contributor.authorGassmann, Reto
dc.contributor.authorTelley, Ivo Andreas
dc.contributor.authorCarvalho, Ana Xavier
dc.date.accessioned2017-01-12T16:51:31Z
dc.date.available2017-06-06T00:30:09Z
dc.date.issued2016-12-19
dc.description.abstractCytokinesis in animal cells requires the constriction of an actomyosin contractile ring, whose architecture and mechanism remain poorly understood. We use laser microsurgery to explore the biophysical properties of constricting rings in Caenorhabditis elegans embryos. Laser cutting causes rings to snap open. However, instead of disintegrating, ring topology recovers and constriction proceeds. In response to severing, a finite gap forms and is repaired by recruitment of new material in an actin polymerization-dependent manner. An open ring is able to constrict, and rings repair from successive cuts. After gap repair, an increase in constriction velocity allows cytokinesis to complete at the same time as controls. Our analysis demonstrates that tension in the ring increases while net cortical tension at the site of ingression decreases throughout constriction and suggests that cytokinesis is accomplished by contractile modules that assemble and contract autonomously, enabling local repair of the actomyosin network. Consequently, cytokinesis is a highly robust process impervious to discontinuities in contractile ring structure.pt_PT
dc.description.sponsorshipEuropean Research Council grant: (640553); Fundo Europeu de Desenvolvimento Regional (FEDER) funds: (Operational Competitiveness Program - COMPETE); Fundação para a Ciência e a Tecnologia grant:(NORTE-07-0124-FEDER-000003); Fundação Luso-Americana para o Desenvolvimento (Life Science 2020); Louis-Jeantet Young Investigator Award; European Social Fund (Programa Operacional Temático Potencial Type 4.2); Programa Operacional Regional do Norte (Quadro de Referência Estratégico Nacional - FEDER).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRobust gap repair in the contractile ring ensures timely completion of cytokinesis Ana M. Silva, Daniel S. Osório, Antonio J. Pereira, Helder Maiato, Inês Mendes Pinto, Boris Rubinstein, Reto Gassmann, Ivo Andreas Telley, Ana Xavier Carvalho J Cell Biol Dec 2016, 215 (6) 789-799; DOI: 10.1083/jcb.201605080pt_PT
dc.identifier.doi10.1083/jcb.201605080pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/727
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRockefeller University Presspt_PT
dc.relationSpatiotemporal regulation of chromosome segregation fidelity
dc.relationCytoplasmic Dynein: Mechanisms of Regulation and Novel Interactors
dc.relationActin and myosin II in the contractile ring - mechanisms of constriction
dc.relationContractile Ring Dynamics and Mechanism of Constriction During Cytokinesis
dc.relation.publisherversionhttp://jcb.rupress.org/content/215/6/789pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectcytokinesispt_PT
dc.subjectCaenorhabditis elegans embryospt_PT
dc.subjectring constrictionpt_PT
dc.titleRobust gap repair in the contractile ring ensures timely completion of cytokinesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSpatiotemporal regulation of chromosome segregation fidelity
oaire.awardTitleCytoplasmic Dynein: Mechanisms of Regulation and Novel Interactors
oaire.awardTitleActin and myosin II in the contractile ring - mechanisms of constriction
oaire.awardTitleContractile Ring Dynamics and Mechanism of Constriction During Cytokinesis
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260892/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/338410/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FBEX-BCM%2F0654%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F95707%2F2013/PT
oaire.citation.endPage799pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage789pt_PT
oaire.citation.titleJournal of Cell Biologypt_PT
oaire.citation.volume215pt_PT
oaire.fundingStreamFP7
oaire.fundingStreamFP7
oaire.fundingStream5876-PPCDTI
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
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relation.isProjectOfPublicationf98218ac-5a3a-4113-bff0-1248e8d2275c
relation.isProjectOfPublication963fa634-d03c-4213-a997-e8347320964b
relation.isProjectOfPublicationcc7be9e4-82fe-4f61-89b8-efc6bf38ccbd
relation.isProjectOfPublication.latestForDiscovery64734181-d82d-45ab-a260-698d3b3be8c1

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