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Requirement for highly efficient pre-mRNA splicing duringDrosophilaearly embryonic development

2014-04-22Journal article Open access
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dc.contributor.authorGuilgur, Leonardo Gastón
dc.contributor.authorPrudêncio, Pedro
dc.contributor.authorSobral, Daniel
dc.contributor.authorLiszekova, Denisa
dc.contributor.authorRosa, André
dc.contributor.authorMartinho, Rui Gonçalo
dc.date.accessioned2015-11-24T18:42:21Z
dc.date.available2015-11-24T18:42:21Z
dc.date.issued2014-04-22
dc.description.abstractDrosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being splicing avoidance a possible explanation why most early zygotic genes are intronless. We isolated two mutant alleles for a subunit of the NTC/Prp19 complexes, which specifically impaired pre-mRNA splicing of early zygotic but not maternally encoded transcripts. We hypothesized that the requirements for pre-mRNA splicing efficiency were likely to vary during development. Ectopic maternal expression of an early zygotic pre-mRNA was sufficient to suppress its splicing defects in the mutant background. Furthermore, a small early zygotic transcript with multiple introns was poorly spliced in wild-type embryos. Our findings demonstrate for the first time the existence of a developmental pre-requisite for highly efficient splicing during Drosophila early embryonic development and suggest in highly proliferative tissues a need for coordination between cell cycle and gene architecture to ensure correct gene expression and avoid abnormally processed transcripts. DOI: http://dx.doi.org/10.7554/eLife.02181.001.pt_PT
dc.description.sponsorshipFCT grant: (PEst-OE/EQB/LA0023/2013), FCT fellowships: (SFRH/BPD/47957/2008, SFRH/BPD/63869/2009).pt_PT
dc.identifier.citationeLife 2014;3:e02181pt_PT
dc.identifier.doi10.7554/eLife.02181pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/524
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElife Sciences Publicationspt_PT
dc.relationCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
dc.relationProtein targeting and N-terminal acetylation
dc.relation.publisherversionhttp://elifesciences.org/content/3/e02181pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPrp19/NTCpt_PT
dc.subjectSyf1/Xab2pt_PT
dc.subjectearly zygotic genespt_PT
dc.subjectfast developmentpt_PT
dc.subjectmRNA splicingpt_PT
dc.titleRequirement for highly efficient pre-mRNA splicing duringDrosophilaearly embryonic developmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
oaire.awardTitleProtein targeting and N-terminal acetylation
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BID%2F111796%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F111822%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F0712%2F2012/PT
oaire.citation.endPage21pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleeLIFEpt_PT
oaire.citation.volume3pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication0009a263-b159-4ca6-98d2-57a0e00de19e
relation.isProjectOfPublication65049509-fca6-473b-90cd-f2ae8072ff4e
relation.isProjectOfPublication98e528d4-77df-4730-9aa7-3462367b024e
relation.isProjectOfPublication.latestForDiscovery0009a263-b159-4ca6-98d2-57a0e00de19e

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