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Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

2019Journal article Open access
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dc.contributor.authorAlenquer, Marta
dc.contributor.authorVale-Costa, Sílvia
dc.contributor.authorEtibor, Temitope Akhigbe
dc.contributor.authorFerreira, Filipe
dc.contributor.authorSousa, Ana Laura
dc.contributor.authorAmorim, Maria João
dc.date.accessioned2020-03-06T15:35:13Z
dc.date.available2020-03-06T15:35:13Z
dc.date.issued2019
dc.description.abstractInfluenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic reticulum (ER) exit sites, depend on continuous ER-Golgi vesicular cycling and do not promote escape to interferon response. We propose that viral inclusions segregate vRNPs from the cytosol and facilitate selected RNA-RNA interactions in a liquid environment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41467-019-09549-4pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/916
dc.language.isoengpt_PT
dc.relationIF/00899/2013pt_PT
dc.relationExploring the role and dynamics of skip/plekhm2 in influenza A virus infection.
dc.subjectA549 Cellspt_PT
dc.subjectAnimalspt_PT
dc.subjectCytosolpt_PT
dc.subjectDogspt_PT
dc.subjectEndoplasmic Reticulumpt_PT
dc.subjectGolgi Apparatuspt_PT
dc.subjectHEK293 Cellspt_PT
dc.subjectHeLa Cellspt_PT
dc.subjectHost-Pathogen Interactionspt_PT
dc.subjectHumanspt_PT
dc.subjectInfluenza A viruspt_PT
dc.subjectInfluenza, Humanpt_PT
dc.subjectMadin Darby Canine Kidney Cellspt_PT
dc.subjectProtein Bindingpt_PT
dc.subjectRNA, Viralpt_PT
dc.subjectRibonucleoproteinspt_PT
dc.subjectViral Proteinspt_PT
dc.subjectrab GTP-Binding Proteinspt_PT
dc.subjectVirus Assemblypt_PT
dc.titleInfluenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sitespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleExploring the role and dynamics of skip/plekhm2 in influenza A virus infection.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-CEL%2F32211%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F128436%2F2017/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1629pt_PT
oaire.citation.titleNature Communicationspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication78e8d97a-0227-4267-bae9-478c27b9bc41
relation.isProjectOfPublication76e474ba-554d-4005-b2b4-5aa1d4323bf0
relation.isProjectOfPublication.latestForDiscovery78e8d97a-0227-4267-bae9-478c27b9bc41

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