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ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis

2013-10Journal article Open access
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dc.contributor.authorRodrigues, A.
dc.contributor.authorAdamo, M.
dc.contributor.authorCrozet, P.
dc.contributor.authorMargalha, L.
dc.contributor.authorConfraria, A.
dc.contributor.authorMartinho, C.
dc.contributor.authorElias, A.
dc.contributor.authorRabissi, A.
dc.contributor.authorLumbreras, V.
dc.contributor.authorGonzalez-Guzman, M.
dc.contributor.authorAntoni, R.
dc.contributor.authorRodriguez, P. L.
dc.contributor.authorBaena-Gonzalez, E.
dc.date.accessioned2016-06-07T11:25:30Z
dc.date.available2016-06-07T11:25:30Z
dc.date.issued2013-10
dc.description.abstractPlant survival under environmental stress requires the integration of multiple signaling pathways into a coordinated response, but the molecular mechanisms underlying this integration are poorly understood. Stress-derived energy deprivation activates the Snf1-related protein kinases1 (SnRK1s), triggering a vast transcriptional and metabolic reprogramming that restores homeostasis and promotes tolerance to adverse conditions. Here, we show that two clade A type 2C protein phosphatases (PP2Cs), established repressors of the abscisic acid (ABA) hormonal pathway, interact with the SnRK1 catalytic subunit causing its dephosphorylation and inactivation. Accordingly, SnRK1 repression is abrogated in double and quadruple pp2c knockout mutants, provoking, similarly to SnRK1 overexpression, sugar hypersensitivity during early seedling development. Reporter gene assays and SnRK1 target gene expression analyses further demonstrate that PP2C inhibition by ABA results in SnRK1 activation, promoting SnRK1 signaling during stress and once the energy deficit subsides. Consistent with this, SnRK1 and ABA induce largely overlapping transcriptional responses. Hence, the PP2C hub allows the coordinated activation of ABA and energy signaling, strengthening the stress response through the cooperation of two key and complementary pathways.pt_PT
dc.description.sponsorshipMarie Curie IRG grants; the EMBO Installation program; Marie Curie Actions grant: (FP7-People-2010-ITN); FCT grants: (PTDC/AGR-AAM/104939/2008, SFRH/BPD/47280/2008, SFRH/BD/33563/2008, SFRH/BD/51627/2011, SFRH/BPD/79255/2011); Portugal-Spain Bilateral Collaboration program Ações integradas: (Ação E-26/10); Generalitat de Catalunya PhD grant: (FI-AR067443); Ministerio de Ciencia e Innovación grants: (BIO2011-23446, PT2009-0155); Junta para Ampliación de Estudios e Investigaciones Científicas-Consejo Superior de Investigaciones Cientificas fellowship; Juan de la Cierva contract.pt_PT
dc.identifier.citationAmérico Rodrigues, Mattia Adamo, Pierre Crozet, Leonor Margalha, Ana Confraria, Cláudia Martinho, Alexandre Elias, Agnese Rabissi, Victoria Lumbreras, Miguel González-Guzmán, Regina Antoni, Pedro L. Rodriguez, and Elena Baena-González ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis Plant Cell 2013 25: 3871-3884. Advance Publication October 31, 2013; doi:10.1105/tpc.113.114066pt_PT
dc.identifier.doi10.1105/tpc.113.114066pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/621
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society of Plant Physiologistspt_PT
dc.relation.publisherversionhttp://www.plantcell.org/content/25/10/3871.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAbscisic Acidpt_PT
dc.subjectArabidopsispt_PT
dc.subjectArabidopsis Proteinspt_PT
dc.subjectGene Expression Regulation, Plantpt_PT
dc.subjectPhosphoprotein Phosphatasespt_PT
dc.subjectPhosphorylationpt_PT
dc.subjectPlant Growth Regulatorspt_PT
dc.subjectProtein-Serine-Threonine Kinasespt_PT
dc.subjectStress, Physiologicalpt_PT
dc.subjectSignal Transductionpt_PT
dc.titleABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3884pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage3871pt_PT
oaire.citation.titleThe Plant Cellpt_PT
oaire.citation.volume25pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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