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SUMOylation represses SnRK1 signaling in Arabidopsis

2015-12-30Journal article Open access
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dc.contributor.authorCrozet, Pierre
dc.contributor.authorMargalha, Leonor
dc.contributor.authorButowt, Rafal
dc.contributor.authorFernandes, Noémia
dc.contributor.authorElias, Carlos A.
dc.contributor.authorOrosa, Beatriz
dc.contributor.authorTomanov, Konstantin
dc.contributor.authorTeige, Markus
dc.contributor.authorBachmair, Andreas
dc.contributor.authorSadanandom, Ari
dc.contributor.authorBaena-González, Elena
dc.date.accessioned2016-06-07T11:56:07Z
dc.date.available2016-12-01T01:30:10Z
dc.date.issued2015-12-30
dc.description.abstractThe SnRK1 protein kinase balances cellular energy levels in accordance with extracellular conditions and is thereby key for plant stress tolerance. In addition, SnRK1 has been implicated in numerous growth and developmental processes from seed filling and maturation to flowering and senescence. Despite its importance, the mechanisms that regulate SnRK1 activity are poorly understood. Here, we demonstrate that the SnRK1 complex is SUMOylated on multiple subunits and identify SIZ1 as the E3 Small Ubiquitin-like Modifier (SUMO) ligase responsible for this modification. We further show that SnRK1 is ubiquitinated in a SIZ1-dependent manner, causing its degradation through the proteasome. In consequence, SnRK1 degradation is deficient in siz1-2 mutants, leading to its accumulation and hyperactivation of SnRK1 signaling. Finally, SnRK1 degradation is strictly dependent on its activity, as inactive SnRK1 variants are aberrantly stable but recover normal degradation when expressed as SUMO mimetics. Altogether, our data suggest that active SnRK1 triggers its own SUMOylation and degradation, establishing a negative feedback loop that attenuates SnRK1 signaling and prevents detrimental hyperactivation of stress responses.pt_PT
dc.description.sponsorshipAustrian Science Foundation FWF grant: (P25488, P23435); EMBO Installation program; FCT grants: (PTDC/BIA-PLA/3937/2012, SFRH/BD/51627/2011,SFRH/BPD/79255/2011); UID/Multi/04551/2013_Research unit GREEN-it_'Bioresources for Sustainability’.pt_PT
dc.identifier.citationCrozet, P., Margalha, L., Butowt, R., Fernandes, N., Elias, C. A., Orosa, B., Tomanov, K., Teige, M., Bachmair, A., Sadanandom, A. and Baena-González, E. (2016), SUMOylation represses SnRK1 signaling in Arabidopsis. Plant J, 85: 120–133. doi:10.1111/tpj.13096pt_PT
dc.identifier.doi10.1111/tpj.13096pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/623
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.13096/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSUMOylationpt_PT
dc.subjectubiquitinationpt_PT
dc.subjectSNF1-related protein kinase (SnRK1)pt_PT
dc.subjectenergy 18 signalingpt_PT
dc.subjectstresspt_PT
dc.subjectSIZ1pt_PT
dc.subjectArabidopsis thalianapt_PT
dc.titleSUMOylation represses SnRK1 signaling in Arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage133pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage120pt_PT
oaire.citation.titleThe Plant Journalpt_PT
oaire.citation.volume85pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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