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Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing

2015-10-29Journal article Open access
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dc.contributor.authorGarelli, Andres
dc.contributor.authorHeredia, Fabiana
dc.contributor.authorCasimiro, Andreia P.
dc.contributor.authorMacedo, Andre
dc.contributor.authorNunes, Catarina
dc.contributor.authorGarcez, Marcia
dc.contributor.authorDias, Angela R. Mantas
dc.contributor.authorVolonte, Yanel A.
dc.contributor.authorUhlmann, Thomas
dc.contributor.authorCaparros, Esther
dc.contributor.authorKoyama, Takashi
dc.contributor.authorGontijo, Alisson M.
dc.date.accessioned2015-11-18T12:01:52Z
dc.date.available2015-11-18T12:01:52Z
dc.date.issued2015-10-29
dc.description.abstractHow different organs in the body sense growth perturbations in distant tissues to coordinate their size during development is poorly understood. Here we mutate an invertebrate orphan relaxin receptor gene, the Drosophila Leucine-rich repeat-containing G protein-coupled receptor 3 (Lgr3), and find body asymmetries similar to those found in insulin-like peptide 8 (dilp8) mutants, which fail to coordinate growth with developmental timing. Indeed, mutation or RNA intereference (RNAi) against Lgr3 suppresses the delay in pupariation induced by imaginal disc growth perturbation or ectopic Dilp8 expression. By tagging endogenous Lgr3 and performing cell type-specific RNAi, we map this Lgr3 activity to a new subset of CNS neurons, four of which are a pair of bilateral pars intercerebralis Lgr3-positive (PIL) neurons that respond specifically to ectopic Dilp8 by increasing cAMP-dependent signalling. Our work sheds new light on the function and evolution of relaxin receptors and reveals a novel neuroendocrine circuit responsive to growth aberrations.pt_PT
dc.description.sponsorshipFCT Investigator Programme: (PD/BD/52421/2013), FCT fellowship: (SFRH/BPD/94112/2013, SFRH/BD/94931/2013, SFRH/BPD/74313/2010), CONICET and UNS, CONICET fellowship, CEDOC, N.P. Rotstein and L.E. Politi.pt_PT
dc.identifier.citationGarelli, A. et al. Dilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timing. Nat. Commun. 6:8732 doi: 10.1038/ ncomms9732 (2015).pt_PT
dc.identifier.doi10.1038/ncomms9732pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/499
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationThe molecular physiology and the evolution of a new pathway promoting developmental stability
dc.relation.publisherversionhttp://www.nature.com/ncomms/2015/151029/ncomms9732/full/ncomms9732.htmlpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiological sciencespt_PT
dc.subjectCell biologypt_PT
dc.subjectNeurosciencept_PT
dc.titleDilp8 requires the neuronal relaxin receptor Lgr3 to couple growth to developmental timingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleThe molecular physiology and the evolution of a new pathway promoting developmental stability
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/618847/EU
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleNature Communicationspt_PT
oaire.citation.volume6pt_PT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication11c7ee1e-fd47-462e-a249-35ba586ee591
relation.isProjectOfPublication.latestForDiscovery11c7ee1e-fd47-462e-a249-35ba586ee591

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