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Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings

2016-02Book part Open access
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dc.contributor.authorRemy, Estelle
dc.contributor.authorDuque, Paula
dc.date.accessioned2018-04-13T11:33:23Z
dc.date.available2018-04-13T11:33:23Z
dc.date.issued2016-02
dc.descriptionThe deposited book chapter is a post-print version and has been submitted to peer review.pt_PT
dc.descriptionThe deposited book chapter version contains attached the supplementary materials within the pdf.pt_PT
dc.descriptionThis publication hasn't any creative commons license associated.pt_PT
dc.descriptionThe deposited book chapter is part of the book series: "Environmental Responses in Plants: Methods and Protocols" (pp.197-208) published by Springer.pt_PT
dc.description.abstractHeavy-metal soil contamination is one of the major abiotic stress factors that, by negatively affecting plant growth and development, severely limit agricultural productivity worldwide. Plants have evolved various tolerance and detoxification strategies in order to cope with heavy-metal toxicity while ensuring adequate supply of essential micronutrients at the whole-plant as well as cellular levels. Genetic studies in the model plant Arabidopsis thaliana have been instrumental in elucidating such mechanisms. The root assay constitutes a very powerful and simple method to assess heavy-metal stress tolerance in Arabidopsis seedlings. It allows the simultaneous determination of all the standard growth parameters affected by heavy-metal stress (primary root elongation, lateral root development, shoot biomass, and chlorophyll content) in a single experiment. Additionally, this protocol emphasizes the tips and tricks that become particularly useful when quantifying subtle alterations in tolerance to a given heavy-metal stress, when simultaneously pursuing a large number of plant lines, or when testing sensitivity to a wide range of heavy metals for a single line.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grants: (EXPL/AGR-PRO/1013/2013, SFRH/BPD/44640/2008); GREEN-it "Bioresources for Sustainability": (UID/Multi/04551/2013).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRemy,E., Duque, P. (2016). Methods Mol.Biol. 1398:197-208. doi: 10.1007/978-1-4939-3356-3_16.pt_PT
dc.identifier.doi10.1007/978-1-4939-3356-3_16pt_PT
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.urihttp://hdl.handle.net/10400.7/865
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Publishing Companypt_PT
dc.relationMOLECULAR ANALYSIS OF HERBICIDE RESISTANCE IN ARABIDOPSIS THALIANA
dc.relation.publisherversionhttps://journals.cshl.edu:2443/login?qurl=https%3a%2f%2flink.springer.com%2f10.1007%2f978-1-4939-3356-3_16pt_PT
dc.subjectArabidopsis thalianapt_PT
dc.subjectChlorophyll contentpt_PT
dc.subjectHeavy-metal stress tolerancept_PT
dc.subjectLateral root developmentpt_PT
dc.subjectPrimary root elongationpt_PT
dc.subjectRoot assaypt_PT
dc.subjectSeedlingspt_PT
dc.subjectShoot biomasspt_PT
dc.titleAssessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlingspt_PT
dc.typebook part
dspace.entity.typePublication
oaire.awardTitleMOLECULAR ANALYSIS OF HERBICIDE RESISTANCE IN ARABIDOPSIS THALIANA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FAGR-PRO%2F1013%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44640%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04551%2F2013/PT
oaire.citation.endPage208pt_PT
oaire.citation.startPage197pt_PT
oaire.citation.titleMethods in Molecular Biologypt_PT
oaire.citation.volume1398pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typebookPartpt_PT
relation.isProjectOfPublicationb66a8488-68e7-452c-a018-223ce17fb469
relation.isProjectOfPublication50a427d5-4b52-497a-9718-027fa571eb81
relation.isProjectOfPublicationf0a3f9cf-be53-424f-ac33-17539df53098
relation.isProjectOfPublication.latestForDiscoveryb66a8488-68e7-452c-a018-223ce17fb469

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