Browsing by Author "Kombila, M."
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- Self-reactivities to the non-erythroid alpha spectrin correlate with cerebral malaria in Gabonese childrenPublication . Guiyedi, V.; Chanseaud, Y.; Fesel, C.; Snounou, G.; Rousselle, J.C.; Lim, P.; Koko, J.; Namane, A.; Cazenave, P.A.; Kombila, M.; Pied, S.Hypergammaglobulinemia and polyclonal B-cell activation commonly occur in Plasmodium sp. infections. Some of the antibodies produced recognize self-components and are correlated with disease severity in P. falciparum malaria. However, it is not known whether some self-reactive antibodies produced during P. falciparum infection contribute to the events leading to cerebral malaria (CM). We show here a correlation between self-antibody responses to a human brain protein and high levels of circulating TNF alpha (TNFα), with the manifestation of CM in Gabonese children
- Total and functional parasite specific IgE responses in Plasmodium falciparum-infected patients exhibiting different clinical statusPublication . Duarte, J.; Deshpande, P.; Guiyedi, V.; Mecheri, S.; Fesel, C.; Cazenave, P.A.; Mishra, G.C.; Kombila, M.; Pied, S.Blood samples were collected from controls and P. falciparum-infected patients before treatment on the day of hospitalization (day 0) in India and, in addition, on days 7 and 30 after treatment in Gabon. Total IgE levels were determined by ELISA and functional P. falciparum-specific IgE were estimated using a mast cell line RBL-2H3 transfected with a human Fcε RI α-chain that triggers degranulation upon human IgE cross-linking. Mann Whitney and Kruskall Wallis tests were used to compare groups and the Spearman test was used for correlations. Total IgE levels were confirmed to increase upon infection and differ with level of transmission and age but were not directly related to the disease phenotype. All studied groups exhibited functional parasite-specific IgEs able to induce mast cell degranulation in vitro in the presence of P. falciparum antigens. Plasma IgE levels correlated with those of IL-10 in uncomplicated malaria patients from Gabon. In Indian patients, plasma IFN-γ , TNF and IL-10 levels were significantly correlated with IgE concentrations in all groups.