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Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling

2016-08-10Journal article Open access
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dc.contributor.authorNavarro-Costa, Paulo
dc.contributor.authorMcCarthy, Alicia
dc.contributor.authorPrudêncio, Pedro
dc.contributor.authorGreer, Christina
dc.contributor.authorGuilgur, Leonardo G.
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorSecombe, Julie
dc.contributor.authorRangan, Prashanth
dc.contributor.authorMartinho, Rui G.
dc.date.accessioned2016-08-23T15:06:46Z
dc.date.available2016-08-23T15:06:46Z
dc.date.issued2016-08-10
dc.description.abstractOocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I.pt_PT
dc.description.sponsorshipHarvard Medical School; NIH/NIGMS grants: R01-GM084947, RO1 1119484-1-68857, R01GM112783); Fundação para a Ciência e a Tecnologia grant: (PTDC/BEX-BID/0395/2014); SUNY Presidential Innovative Award; Pew Biomedical Scholars Program.pt_PT
dc.identifier.citationNavarro-Costa, P. et al. Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling. Nat. Commun. 7:12331 doi: 10.1038/ncomms12331 (2016).pt_PT
dc.identifier.doi10.1038/ncomms12331pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/693
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationProtein targeting and N-terminal acetylation
dc.relationIDENTIFICATION AND CHARACTERIZATION OF NOVEL DROSOPHILA GERMLINE GENES
dc.relation.publisherversionhttp://www.nature.com/articles/ncomms12331pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectEpigeneticspt_PT
dc.subjectmeiosispt_PT
dc.titleEarly programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodellingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleProtein targeting and N-terminal acetylation
oaire.awardTitleIDENTIFICATION AND CHARACTERIZATION OF NOVEL DROSOPHILA GERMLINE GENES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F0712%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F84214%2F2012/PT
oaire.citation.endPage15pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleNature Communicationspt_PT
oaire.citation.volume7pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication98e528d4-77df-4730-9aa7-3462367b024e
relation.isProjectOfPublication6e63cd83-1086-4cdb-bd52-38999aba5657
relation.isProjectOfPublication4bc05b5e-75b8-435a-ac4f-25a939a54c9e
relation.isProjectOfPublication.latestForDiscovery4bc05b5e-75b8-435a-ac4f-25a939a54c9e

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