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Rates of transposition in Escherichia coli

2013-12-04Journal article Open access
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dc.contributor.authorSousa, A.
dc.contributor.authorBourgard, C.
dc.contributor.authorWahl, L. M.
dc.contributor.authorGordo, I.
dc.date.accessioned2015-12-22T15:33:57Z
dc.date.available2015-12-22T15:33:57Z
dc.date.issued2013-12-04
dc.description.abstractThe evolutionary role of transposable elements (TEs) is still highly controversial. Two key parameters, the transposition rate (u and w, for replicative and non-replicative transposition) and the excision rate (e) are fundamental to understanding their evolution and maintenance in populations. We have estimated u, w and e for six families of TEs (including eight members: IS1, IS2, IS3, IS4, IS5, IS30, IS150 and IS186) in Escherichia coli, using a mutation accumulation (MA) experiment. In this experiment, mutations accumulate essentially at the rate at which they appear, during a period of 80 500 (1610 generations × 50 lines) generations, and spontaneous transposition events can be detected. This differs from other experiments in which insertions accumulated under strong selective pressure or over a limited genomic target. We therefore provide new estimates for the spontaneous rates of transposition and excision in E. coli. We observed 25 transposition and three excision events in 50 MA lines, leading to overall rate estimates of u ∼ 1.15 × 10(-5), w ∼ 4 × 10(-8) and e ∼ 1.08 × 10(-6) (per element, per generation). Furthermore, extensive variation between elements was found, consistent with previous knowledge of the mechanisms and regulation of transposition for the different elements.pt_PT
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada, LAO/ITQB, FCT.pt_PT
dc.identifier.citationAna Sousa, Catarina Bourgard, Lindi M. Wahl, Isabel Gordo Biol. Lett. 2013 9 20130838; DOI: 10.1098/rsbl.2013.0838. Published 4 December 2013pt_PT
dc.identifier.doi10.1098/rsbl.2013.0838pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/545
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherThe Royal Societypt_PT
dc.relationMicrobial adaptation within ecosystems
dc.relationFitness effects of synonymous mutations
dc.relation.publisherversionhttp://rsbl.royalsocietypublishing.org/content/9/6/20130838.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjecttransposable elementspt_PT
dc.subjectinsertion sequencespt_PT
dc.subjectrate of transpositionpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectmutation accumulationpt_PT
dc.titleRates of transposition in Escherichia colipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardTitleFitness effects of synonymous mutations
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-EVF%2F3247%2F2012/PT
oaire.citation.endPage4pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleBiology Letterspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublicationa8a5dc2f-9213-4e87-90ee-9e0468275b61
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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