Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster

Herboso, Leire; Oliveira, Marisa M.; Talamillo, Ana; Pérez, Coralia; González, Monika; Martín, David; Sutherland, James D.; Shingleton, Alexander W.; Mirth, Christen K.; Barrio, Rosa

Publicação

Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster

2015-07-22Artigo científico

dc.contributor.author	Herboso, Leire
dc.contributor.author	Oliveira, Marisa M.
dc.contributor.author	Talamillo, Ana
dc.contributor.author	Pérez, Coralia
dc.contributor.author	González, Monika
dc.contributor.author	Martín, David
dc.contributor.author	Sutherland, James D.
dc.contributor.author	Shingleton, Alexander W.
dc.contributor.author	Mirth, Christen K.
dc.contributor.author	Barrio, Rosa
dc.date.accessioned	2016-05-05T10:21:57Z
dc.date.available	2016-05-05T10:21:57Z
dc.date.issued	2015-07-22
dc.description.abstract	Animals have a determined species-specific body size that results from the combined action of hormones and signaling pathways regulating growth rate and duration. In Drosophila, the steroid hormone ecdysone controls developmental transitions, thereby regulating the duration of the growth period. Here we show that ecdysone promotes the growth of imaginal discs in mid-third instar larvae, since imaginal discs from larvae with reduced or no ecdysone synthesis are smaller than wild type due to smaller and fewer cells. We show that insulin-like peptides are produced and secreted normally in larvae with reduced ecdysone synthesis, and upstream components of insulin/insulin-like signaling are activated in their discs. Instead, ecdysone appears to regulate the growth of imaginal discs via Thor/4E-BP, a negative growth regulator downstream of the insulin/insulin-like growth factor/Tor pathways. Discs from larvae with reduced ecdysone synthesis have elevated levels of Thor, while mutations in Thor partially rescue their growth. The regulation of organ growth by ecdysone is evolutionarily conserved in hemimetabolous insects, as shown by our results obtained using Blattella germanica. In summary, our data provide new insights into the relationship between components of the insulin/insulin-like/Tor and ecdysone pathways in the control of organ growth.	pt_PT
dc.description.sponsorship	Spanish Ministry of Science and Consolider program grants: (BFU-2008-01884, BFU2011-25986, CSD2007-008-25120, BFU2009-10571 and BES-2009-016077); Departments of Education and Industry of the Basque Government grant: (PI2012/42); Bizkaia County; Instituto Gulbenkian de Ciência/Fundação Calouste Gulbenkian; Fundação Para a Ciência e a Tecnologia fellowship: (SFRH/BD/51181/2010).	pt_PT
dc.identifier.citation	Herboso, L. et al. Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster. Sci. Rep. 5, 12383; doi: 10.1038/srep12383 (2015).	pt_PT
dc.identifier.doi	10.1038/srep12383	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/598
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Nature Publishing Group	pt_PT
dc.relation.publisherversion	http://www.nature.com/articles/srep12383	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Cell proliferation	pt_PT
dc.subject	Development	pt_PT
dc.subject	Limb development	pt_PT
dc.subject	TOR signalling	pt_PT
dc.title	Ecdysone promotes growth of imaginal discs through the regulation of Thor in D. melanogaster	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	14	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	Scientific Reports	pt_PT
oaire.citation.volume	5	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT