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Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance

2017-07-03Journal article Open access
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dc.contributor.authorRemy, Estelle
dc.contributor.authorNiño-González, María
dc.contributor.authorGodinho, Cláudia P.
dc.contributor.authorCabrito, Tânia R.
dc.contributor.authorTeixeira, Miguel C.
dc.contributor.authorSá-Correia, Isabel
dc.contributor.authorDuque, Paula
dc.date.accessioned2017-07-12T14:47:53Z
dc.date.available2017-07-12T14:47:53Z
dc.date.issued2017-07-03
dc.descriptionThis deposit is composed by the main article plus the supplementary materials of the publication.pt_PT
dc.description.abstractSoil contamination is a major hindrance for plant growth and development. The lack of effective strategies to remove chemicals released into the environment has raised the need to increase plant resilience to soil pollutants. Here, we investigated the ability of two Saccharomyces cerevisiae plasma-membrane transporters, the Major Facilitator Superfamily (MFS) member Tpo1p and the ATP-Binding Cassette (ABC) protein Pdr5p, to confer Multiple Drug Resistance (MDR) in Arabidopsis thaliana. Transgenic plants expressing either of the yeast transporters were undistinguishable from the wild type under control conditions, but displayed tolerance when challenged with the herbicides 2,4-D and barban. Plants expressing ScTPO1 were also more resistant to the herbicides alachlor and metolachlor as well as to the fungicide mancozeb and the Co(2+), Cu(2+), Ni(2+), Al(3+) and Cd(2+) cations, while ScPDR5-expressing plants exhibited tolerance to cycloheximide. Yeast mutants lacking Tpo1p or Pdr5p showed increased sensitivity to most of the agents tested in plants. Our results demonstrate that the S. cerevisiae Tpo1p and Pdr5p transporters are able to mediate resistance to a broad range of compounds of agricultural interest in yeast as well as in Arabidopsis, underscoring their potential in future biotechnological applications.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grants: (EXPL/AGR-PRO/1013/2013, PTDC/BIA-PLA/1084/2014, SFRH/BPD/44640/2008, SFRH/BPD/81221/2011, PD/BD/105735/2014, PD/00133/2012, SFRH/BD/92552/2013, UID/BIO/04565/2013, UID/Multi/04551/2013). Programa Operacional Regional de Lisboa 2020 grant: (Project N. 007317).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRemy, E. et al. Heterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerance. Sci Rep 7, 4529 (2017). doi:10.1038/s41598-017-04534-7pt_PT
dc.identifier.doi10.1038/s41598-017-04534-7pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/775
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationPD/00133/2012pt_PT
dc.relationAlternative Splicing and SR Proteins in ABA-mediated Stress Responses
dc.relationMOLECULAR ANALYSIS OF HERBICIDE RESISTANCE IN ARABIDOPSIS THALIANA
dc.relationUNVEILING THE ROLE OF MULTIDRUG RESISTANCE TRANSPORTERS IN CHEMICAL STRESS DEFENCE AT A SYSTEMS LEVEL : FROM THE MODEL ORGANISMS S. CEREVISIAE AND A. THALIANA TO BIOTECHNOLOGICAL APPLICATIONS
dc.relationThe Major Facilitator Superfamily MFS of membrane transporters:Uncovering novel determinants of plant abiotic stress tolerence
dc.relationTHE ROLES OF ABC AND MFS MULTIDRUG RESISTANCE TRANSPORTERS IN THE DEFENCE MECHANISMS OF EUKARYOTIC CELLS:STUDIES IN THE YEAST MODEL
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-017-04534-7pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAbioticpt_PT
dc.subjectMolecular engineering in plantspt_PT
dc.titleHeterologous expression of the yeast Tpo1p or Pdr5p membrane transporters in Arabidopsis confers plant xenobiotic tolerancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlternative Splicing and SR Proteins in ABA-mediated Stress Responses
oaire.awardTitleMOLECULAR ANALYSIS OF HERBICIDE RESISTANCE IN ARABIDOPSIS THALIANA
oaire.awardTitleUNVEILING THE ROLE OF MULTIDRUG RESISTANCE TRANSPORTERS IN CHEMICAL STRESS DEFENCE AT A SYSTEMS LEVEL : FROM THE MODEL ORGANISMS S. CEREVISIAE AND A. THALIANA TO BIOTECHNOLOGICAL APPLICATIONS
oaire.awardTitleThe Major Facilitator Superfamily MFS of membrane transporters:Uncovering novel determinants of plant abiotic stress tolerence
oaire.awardTitleTHE ROLES OF ABC AND MFS MULTIDRUG RESISTANCE TRANSPORTERS IN THE DEFENCE MECHANISMS OF EUKARYOTIC CELLS:STUDIES IN THE YEAST MODEL
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FAGR-PRO%2F1013%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-PLA%2F1084%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44640%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F81221%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//PD%2FBD%2F105735%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F92552%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04565%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04551%2F2013/PT
oaire.citation.endPage14pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume7pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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