Publication
The quantitative architecture of centromeric chromatin
| dc.contributor.author | Bodor, Dani L | |
| dc.contributor.author | Mata, João F | |
| dc.contributor.author | Sergeev, Mikhail | |
| dc.contributor.author | David, Ana Filipa | |
| dc.contributor.author | Salimian, Kevan J | |
| dc.contributor.author | Panchenko, Tanya | |
| dc.contributor.author | Cleveland, Don W | |
| dc.contributor.author | Black, Ben E | |
| dc.contributor.author | Shah, Jagesh V | |
| dc.contributor.author | Jansen, Lars ET | |
| dc.date.accessioned | 2015-11-12T16:30:38Z | |
| dc.date.available | 2015-11-12T16:30:38Z | |
| dc.date.issued | 2014-07-15 | |
| dc.description.abstract | The centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and epigenetic inheritance of centromeres. Using complementary strategies, we determined that typical human centromeres contain ∼400 molecules of CENP-A, which is controlled by a mass-action mechanism. This number, despite representing only ∼4% of all centromeric nucleosomes, forms a ∼50-fold enrichment to the overall genome. In addition, although pre-assembled CENP-A is randomly segregated during cell division, this amount of CENP-A is sufficient to prevent stochastic loss of centromere function and identity. Finally, we produced a statistical map of CENP-A occupancy at a human neocentromere and identified nucleosome positions that feature CENP-A in a majority of cells. In summary, we present a quantitative view of the centromere that provides a mechanistic framework for both robust epigenetic inheritance of centromeres and the paucity of neocentromere formation.DOI: http://dx.doi.org/10.7554/eLife.02137.001. | pt_PT |
| dc.description.sponsorship | European Molecular Biology Organization: (EMBO Installation grant), European Commission: (FP7 Marie Curie Reintegration grant), National Institutes of Health grants: (GM082989, GM077238), Burroughs Wellcome Fund, Rita Allen Foundation, Beckman Laser Institute and Foundation, FCT fellowship: (SFRH/BD/74284/2010). | pt_PT |
| dc.identifier.doi | 10.7554/eLife.02137 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.7/492 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elife Sciences Publications | pt_PT |
| dc.relation | Mechanisms of Chromatin-based Epigenetic Inheritance | |
| dc.relation.publisherversion | http://elifesciences.org/content/3/e02137 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | CENP-A | pt_PT |
| dc.subject | centromere | pt_PT |
| dc.subject | epigenetics | pt_PT |
| dc.subject | histone variant | pt_PT |
| dc.subject | molecular counting | pt_PT |
| dc.subject | quantitative microscopy | pt_PT |
| dc.title | The quantitative architecture of centromeric chromatin | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Mechanisms of Chromatin-based Epigenetic Inheritance | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/FP7/615638/EU | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F100557%2F2008/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-PRO%2F100537%2F2008/PT | |
| oaire.citation.endPage | 26 | pt_PT |
| oaire.citation.startPage | 1 | pt_PT |
| oaire.citation.title | eLIFE | pt_PT |
| oaire.fundingStream | FP7 | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 3599-PPCDT | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | European Commission | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | fc0420ff-72cc-4e0d-8521-76c288c7a6a5 | |
| relation.isProjectOfPublication | 920402dc-3fe1-4134-b1c7-1e51333ae32f | |
| relation.isProjectOfPublication | 335e7483-9cd3-449a-b37a-bea73b224517 | |
| relation.isProjectOfPublication.latestForDiscovery | fc0420ff-72cc-4e0d-8521-76c288c7a6a5 |