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Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm

2011-05-06Journal article Open access
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dc.contributor.authorGoncalves, Lisa
dc.contributor.authorFilipe, Mario
dc.contributor.authorMarques, Sara
dc.contributor.authorSalgueiro, Ana-Marisa
dc.contributor.authorBecker, Jorg D.
dc.contributor.authorBelo, Jose Antonio
dc.date.accessioned2016-08-18T14:40:35Z
dc.date.available2016-08-18T14:40:35Z
dc.date.issued2011-05-06
dc.description.abstractDuring early vertebrate development, the correct establishment of the body axes is critical. The anterior pole of the mouse embryo is established when Distal Visceral Endoderm (DVE) cells migrate to form the Anterior Visceral Endoderm (AVE). Symmetrical expression of Lefty1, Cer1 and Dkk1 determines the direction of DVE migration and the future anterior side. In addition to the establishment of the Anterior-Posterior axis, the AVE has also been implicated in anterior neural specification. To better understand the role of the AVE in these processes, we have performed a differential screening using Affymetrix GeneChip technology with AVE cells isolated from cer1P-EGFP transgenic mouse embryos. We found 175 genes which were upregulated in the AVE and 36 genes in the Proximal-posterior sample. Using DAVID software, we characterized the AVE cell population regarding cellular component, molecular function and biological processes. Among the genes that were found to be upregulated in the AVE, several novel genes were identified. Four of these transcripts displaying high-fold change in the AVE were further characterized by in situ hybridization in early stages of development in order to validate the screening. From those four selected genes, one, denominated Adtk1, was chosen to be functionally characterized by targeted inactivation in ES cells. Adtk1 encodes for a serine/threonine kinase. Adtk1 null mutants are smaller and present short limbs due to decreased mineralization, suggesting a potential role in chondrogenesis during limb development. Taken together, these data point to the importance of reporting novel genes present in the AVE.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia PhD fellowships and research grants; Instituto Gulbenkian de Ciência; Fundação Calouste Gulbenkian; IBB/CBME.pt_PT
dc.identifier.citationGonçalves, L., Filipe, M., Marques, S., Salgueiro, A.-M., Becker, J. D., Belo, J. A. (2011). Identification and functional analysis of novel genes expressed in the Anterior Visceral Endoderm. Int. J. Dev. Biol., 55(3), 281–295.pt_PT
dc.identifier.doi10.1387/ijdb.103273lgpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/692
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherUniversity of the Basque Country Presspt_PT
dc.relation.publisherversionhttp://www.ijdb.ehu.es/web/paper.php?doi=10.1387/ijdb.103273lgpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAmino Acid Sequencept_PT
dc.subjectAnimalspt_PT
dc.subjectCell Movementpt_PT
dc.subjectChondrogenesispt_PT
dc.subjectCytokinespt_PT
dc.subjectEmbryo, Mammalianpt_PT
dc.subjectIntercellular Signaling Peptides and Proteinspt_PT
dc.subjectLeft-Right Determination Factorspt_PT
dc.subjectMicept_PT
dc.subjectMice, Inbred C57BLpt_PT
dc.subjectMice, Transgenicpt_PT
dc.subjectProtein-Serine-Threonine Kinasespt_PT
dc.subjectSequence Alignmentpt_PT
dc.subjectBody Patterningpt_PT
dc.subjectEndodermpt_PT
dc.subjectGene Expression Regulation, Developmentalpt_PT
dc.titleIdentification and functional analysis of novel genes expressed in the Anterior Visceral Endodermpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage295pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage281pt_PT
oaire.citation.titleInternational Journal of Developmental Biologypt_PT
oaire.citation.volume55pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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