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Comparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase bceF Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulence

2013-05Journal article Open access
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dc.contributor.authorFerreira, A. S.
dc.contributor.authorSilva, I. N.
dc.contributor.authorOliveira, V. H.
dc.contributor.authorBecker, J. D.
dc.contributor.authorGivskov, M.
dc.contributor.authorRyan, R. P.
dc.contributor.authorFernandes, F.
dc.contributor.authorMoreira, L. M.
dc.date.accessioned2015-10-29T09:46:55Z
dc.date.available2015-10-29T09:46:55Z
dc.date.issued2013-05
dc.description.abstractThe bacterial tyrosine-kinase (BY-kinase) family comprises the major group of bacterial enzymes endowed with tyrosine kinase activity. We previously showed that the BceF protein from Burkholderia cepacia IST408 belongs to this BY-kinase family and is involved in the biosynthesis of the exopolysaccharide cepacian. However, little is known about the extent of regulation of this protein kinase activity. In order to examine this regulation, we performed a comparative transcriptome profile between the bceF mutant and wild-type B. cepacia IST408. The analyses led to identification of 630 genes whose expression was significantly changed. Genes with decreased expression in the bceF mutant were related to stress response, motility, cell adhesion, and carbon and energy metabolism. Genes with increased expression were related to intracellular signaling and lipid metabolism. Mutation of bceF led to reduced survival under heat shock and UV light exposure, reduced swimming motility, and alteration in biofilm architecture when grown in vitro. Consistent with some of these phenotypes, the bceF mutant demonstrated elevated levels of cyclic-di-GMP. Furthermore, BceF contributed to the virulence of B. cepacia for larvae of the Greater wax moth, Galleria mellonella. Taken together, BceF appears to play a considerable role in many cellular processes, including biofilm formation and virulence. As homologues of BceF occur in a number of pathogenic and plant-associated Burkholderia strains, the modulation of bacterial behavior through tyrosine kinase activity is most likely a widely occurring phenomenon.pt_PT
dc.description.sponsorshipFCT contract: (PTDC/BIA-MIC/66977/2006), FCT postdoctoral grant and a doctoral grant, Fundação Calouste Gulbenkian scholarship.pt_PT
dc.identifier10.1128/AEM.00222-13
dc.identifier.citationComparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase bceF Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulence Ana S. Ferreira, Inês N. Silva, Vítor H. Oliveira, Jörg D. Becker, Michael Givskov, Robert P. Ryan, Fábio Fernandes, and Leonilde M. Moreira Appl. Environ. Microbiol. May 2013 79:9 3009-3020; Accepted manuscript posted online 22 February 2013, doi:10.1128/AEM.00222-13pt_PT
dc.identifier.doi10.1128/AEM.00222-13
dc.identifier.doi10.1128/AEM.00222-13
dc.identifier.urihttp://hdl.handle.net/10400.7/448
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationKINASE- Phosphoproteomics in Burkholderia to assess the role of tyrosine phosphorylation in virulence determinants expression
dc.relation.publisherversionhttp://aem.asm.org/content/79/9/3009.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBurkholderia cepaciapt_PT
dc.subjectProtein-Tyrosine Kinasespt_PT
dc.subjectStress, Physiologicalpt_PT
dc.subjectBiofilmspt_PT
dc.titleComparative Transcriptomic Analysis of the Burkholderia cepacia Tyrosine Kinase bceF Mutant Reveals a Role in Tolerance to Stress, Biofilm Formation, and Virulencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleKINASE- Phosphoproteomics in Burkholderia to assess the role of tyrosine phosphorylation in virulence determinants expression
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-BIQ%2F118260%2F2010/PT
oaire.citation.endPage3020pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage3009pt_PT
oaire.citation.titleApplied and Environmental Microbiologypt_PT
oaire.citation.volume79pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication53e30784-d919-4008-9d4d-14ce5ac1d024
relation.isProjectOfPublication.latestForDiscovery53e30784-d919-4008-9d4d-14ce5ac1d024

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