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Reprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Small RNA

2012-09-28Journal article Restricted access
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dc.contributor.authorCalarco, Joseph P.
dc.contributor.authorBorges, Filipe
dc.contributor.authorDonoghue, Mark T.A.
dc.contributor.authorVan Ex, Frédéric
dc.contributor.authorJullien, Pauline E.
dc.contributor.authorLopes, Telma
dc.contributor.authorGardner, Rui
dc.contributor.authorBerger, Frédéric
dc.contributor.authorFeijó, José A.
dc.contributor.authorBecker, Jörg D.
dc.contributor.authorMartienssen, Robert A.
dc.date.accessioned2016-11-08T16:49:15Z
dc.date.available2016-11-08T16:49:15Z
dc.date.issued2012-09-28
dc.description.abstractEpigenetic inheritance is more widespread in plants than in mammals, in part because mammals erase epigenetic information by germline reprogramming. We sequenced the methylome of three haploid cell types from developing pollen: the sperm cell, the vegetative cell, and their precursor, the postmeiotic microspore, and found that unlike in mammals the plant germline retains CG and CHG DNA methylation. However, CHH methylation is lost from retrotransposons in microspores and sperm cells and restored by de novo DNA methyltransferase guided by 24 nt small interfering RNA, both in the vegetative nucleus and in the embryo after fertilization. In the vegetative nucleus, CG methylation is lost from targets of DEMETER (DME), REPRESSOR OF SILENCING 1 (ROS1), and their homologs, which include imprinted loci and recurrent epialleles that accumulate corresponding small RNA and are premethylated in sperm. Thus genome reprogramming in pollen contributes to epigenetic inheritance, transposon silencing, and imprinting, guided by small RNA.pt_PT
dc.description.sponsorshipNIH grant: (R01 GM067014); Temasek Lifescience Laboratory; NSERC graduate student fellowship; Fred C. Gloeckner Foundation; Belgian American Educational Foundation postdoctoral fellowship: (Herbert Hoover).pt_PT
dc.identifier.citationJoseph P. Calarco, Filipe Borges, Mark T.A. Donoghue, Frédéric Van Ex, Pauline E. Jullien, Telma Lopes, Rui Gardner, Frédéric Berger, José A. Feijó, Jörg D. Becker, Robert A. Martienssen, Reprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Small RNA, Cell, Volume 151, Issue 1, 28 September 2012, Pages 194-205, ISSN 0092-8674, http://dx.doi.org/10.1016/j.cell.2012.09.001. (http://www.sciencedirect.com/science/article/pii/S0092867412010665)pt_PT
dc.identifier.doi10.1016/j.cell.2012.09.001pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/708
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationGENETIC AND EPIGENETIC MECHANISMS REGULATING SPERM CELL FUNCTIONS DURING DOUBLE FERTILIZATION IN FLOWERING PLANTS
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0092867412010665pt_PT
dc.subjectAnimalspt_PT
dc.subjectArabidopsispt_PT
dc.subjectDNA Transposable Elementspt_PT
dc.subjectMammalspt_PT
dc.subjectPollenpt_PT
dc.subjectRNA, Plantpt_PT
dc.subjectRNA, Small Interferingpt_PT
dc.subjectSeedspt_PT
dc.subjectDNA Methylationpt_PT
dc.subjectEpigenesis, Geneticpt_PT
dc.titleReprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Small RNApt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleGENETIC AND EPIGENETIC MECHANISMS REGULATING SPERM CELL FUNCTIONS DURING DOUBLE FERTILIZATION IN FLOWERING PLANTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-GPL%2F103778%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F103787%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F108044%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F48761%2F2008/PT
oaire.citation.endPage205pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage194pt_PT
oaire.citation.titleCellpt_PT
oaire.citation.volume151pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationa11ecff8-6b1c-4365-815e-bb1502381044
relation.isProjectOfPublication0bd97f4e-e163-40b9-a2df-d42173887119
relation.isProjectOfPublicationd803af5b-5e6a-4ed4-8f84-7ad323f18ae5
relation.isProjectOfPublication9d14cd7d-7c39-45d3-ae58-3932370a639a
relation.isProjectOfPublication.latestForDiscoverya11ecff8-6b1c-4365-815e-bb1502381044

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