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Chromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere

2015-05-08Journal article Open access
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dc.contributor.authorFalk, S. J.
dc.contributor.authorGuo, L. Y.
dc.contributor.authorSekulic, N.
dc.contributor.authorSmoak, E. M.
dc.contributor.authorMani, T.
dc.contributor.authorLogsdon, G. A.
dc.contributor.authorGupta, K.
dc.contributor.authorJansen, L. E. T.
dc.contributor.authorVan Duyne, G. D.
dc.contributor.authorVinogradov, S. A.
dc.contributor.authorLampson, M. A.
dc.contributor.authorBlack, B. E.
dc.date.accessioned2016-06-07T15:52:28Z
dc.date.available2016-06-07T15:52:28Z
dc.date.issued2015-05-08
dc.description.abstractInheritance of each chromosome depends upon its centromere. A histone H3 variant, centromere protein A (CENP-A), is essential for epigenetically marking centromere location. We find that CENP-A is quantitatively retained at the centromere upon which it is initially assembled. CENP-C binds to CENP-A nucleosomes and is a prime candidate to stabilize centromeric chromatin. Using purified components, we find that CENP-C reshapes the octameric histone core of CENP-A nucleosomes, rigidifies both surface and internal nucleosome structure, and modulates terminal DNA to match the loose wrap that is found on native CENP-A nucleosomes at functional human centromeres. Thus, CENP-C affects nucleosome shape and dynamics in a manner analogous to allosteric regulation of enzymes. CENP-C depletion leads to rapid removal of CENP-A from centromeres, indicating their collaboration in maintaining centromere identity.pt_PT
dc.description.sponsorshipNIH grants: (GM082989, CA186430, GM008275, GM008216, GM007229); American Heart Association predoctoral fellowship; American Cancer Society postdoctoral fellowship; NSF grant: (agreement DMR-0944772).pt_PT
dc.identifier.citationCENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere By Samantha J. Falk, Lucie Y. Guo, Nikolina Sekulic, Evan M. Smoak, Tomoyasu Mani, Glennis A. Logsdon, Kushol Gupta, Lars E. T. Jansen, Gregory D. Van Duyne, Sergei A. Vinogradov, Michael A. Lampson, Ben E. Black Science08 May 2015 : 699-703pt_PT
dc.identifier.doi10.1126/science.1259308pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/627
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Association for the Advancement of Sciencept_PT
dc.relationMechanisms of Chromatin-based Epigenetic Inheritance
dc.relation.publisherversionhttp://science.sciencemag.org/content/348/6235/699.longpt_PT
dc.subjectAutoantigenspt_PT
dc.subjectCentromerept_PT
dc.subjectChromosomal Proteins, Non-Histonept_PT
dc.subjectDNApt_PT
dc.subjectEpigenesis, Geneticpt_PT
dc.subjectFluorescence Resonance Energy Transferpt_PT
dc.subjectGene Knockdown Techniquespt_PT
dc.subjectHumanspt_PT
dc.subjectNucleosomespt_PT
dc.subjectProtein Structure, Secondarypt_PT
dc.titleChromosomes. CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromerept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMechanisms of Chromatin-based Epigenetic Inheritance
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/615638/EU
oaire.citation.endPage703pt_PT
oaire.citation.issue6235pt_PT
oaire.citation.startPage699pt_PT
oaire.citation.titleSciencept_PT
oaire.citation.volume348pt_PT
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationfc0420ff-72cc-4e0d-8521-76c288c7a6a5
relation.isProjectOfPublication.latestForDiscoveryfc0420ff-72cc-4e0d-8521-76c288c7a6a5

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