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Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family
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Advisor(s)
Abstract(s)
Gene families often consist of members with diverse expression domains reflecting their functions in a wide variety of tissues. However, how the expression of individual members, and thus their tissue-specific functions, diversified during the course of gene family expansion is not well understood. In this study, we approached this question through the analysis of the duplication history and transcriptional evolution of a rapidly expanding subfamily of insect Ly6 genes. We analyzed different insect genomes and identified seven Ly6 genes that have originated from a single ancestor through sequential duplication within the higher Diptera. We then determined how the original embryonic expression pattern of the founding gene diversified by characterizing its tissue-specific expression in the beetle Tribolium castaneum, the butterfly Bicyclus anynana, and the mosquito Anopheles stephensi and those of its duplicates in three higher dipteran species, representing various stages of the duplication history (Megaselia abdita, Ceratitis capitata, and Drosophila melanogaster). Our results revealed that frequent neofunctionalization episodes contributed to the increased expression breadth of this subfamily and that these events occurred after duplication and speciation events at comparable frequencies. In addition, at each duplication node, we consistently found asymmetric expression divergence. One paralog inherited most of the tissue-specificities of the founder gene, whereas the other paralog evolved drastically reduced expression domains. Our approach attests to the power of combining a well-established duplication history with a comprehensive coverage of representative species in acquiring unequivocal information about the dynamics of gene expression evolution in gene families.
Description
The deposited article version is a "MBE Advance Access" published on March 4, 2015" provided by Oxford University Press, and it contains attached the supplementary materials within the pdf.
The deposited article is a post-print version.
Some supplementary materials are not present in the uploaded version of the article.
The deposited article is a post-print version.
Some supplementary materials are not present in the uploaded version of the article.
Keywords
Animals Embryo, Nonmammalian Evolution, Molecular Gene Duplication Gene Expression Profiling Insects Organ Specificity Phylogeny Species Specificity Gene Expression Regulation Genes, Insect Multigene Family
Citation
Kohtaro Tanaka, Yoan Diekmann, Alexis Hazbun, Assia Hijazi, Barbara Vreede, Fernando Roch, Élio Sucena; Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family, Molecular Biology and Evolution, Volume 32, Issue 7, 1 July 2015, Pages 1730–1747, https://doi.org/10.1093/molbev/msv052
Publisher
Oxford University Press