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CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis

2016-05-09Journal article Open access
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dc.contributor.authorZitouni, Sihem
dc.contributor.authorFrancia, Maria E.
dc.contributor.authorLeal, Filipe
dc.contributor.authorMontenegro Gouveia, Susana
dc.contributor.authorNabais, Catarina
dc.contributor.authorDuarte, Paulo
dc.contributor.authorGilberto, Samuel
dc.contributor.authorBrito, Daniela
dc.contributor.authorMoyer, Tyler
dc.contributor.authorKandels-Lewis, Steffi
dc.contributor.authorOhta, Midori
dc.contributor.authorKitagawa, Daiju
dc.contributor.authorHolland, Andrew J.
dc.contributor.authorKarsenti, Eric
dc.contributor.authorLorca, Thierry
dc.contributor.authorLince-Faria, Mariana
dc.contributor.authorBettencourt-Dias, Mónica
dc.date.accessioned2018-02-07T14:59:32Z
dc.date.available2018-02-07T14:59:32Z
dc.date.issued2016-05-09
dc.descriptionThe deposited article is a post-print version (author's manuscript from PMC and available in PMC 2017 May 9).pt_PT
dc.descriptionThis publication hasn't any creative commons license associated.pt_PT
dc.descriptionThis deposit is composed by the main article and the supplementary materials are present in the publisher's page in the following link: https://www.sciencedirect.com/science/article/pii/S0960982216303001?via%3Dihub#sec4pt_PT
dc.description.abstractCentrioles are essential for the assembly of both centrosomes and cilia. Centriole biogenesis occurs once and only once per cell cycle and is temporally coordinated with cell-cycle progression, ensuring the formation of the right number of centrioles at the right time. The formation of new daughter centrioles is guided by a pre-existing, mother centriole. The proximity between mother and daughter centrioles was proposed to restrict new centriole formation until they separate beyond a critical distance. Paradoxically, mother and daughter centrioles overcome this distance in early mitosis, at a time when triggers for centriole biogenesis Polo-like kinase 4 (PLK4) and its substrate STIL are abundant. Here we show that in mitosis, the mitotic kinase CDK1-CyclinB binds STIL and prevents formation of the PLK4-STIL complex and STIL phosphorylation by PLK4, thus inhibiting untimely onset of centriole biogenesis. After CDK1-CyclinB inactivation upon mitotic exit, PLK4 can bind and phosphorylate STIL in G1, allowing pro-centriole assembly in the subsequent S phase. Our work shows that complementary mechanisms, such as mother-daughter centriole proximity and CDK1-CyclinB interaction with centriolar components, ensure that centriole biogenesis occurs once and only once per cell cycle, raising parallels to the cell-cycle regulation of DNA replication and centromere formation.pt_PT
dc.description.sponsorshipERC grant: (ERC-2010-StG-261344); FCT grants: (FCT Investigator, EXPL/BIM-ONC/0830/2013, PTDC/SAU-BD/105616/2008); EMBO installation grant.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSihem Zitouni, Maria E. Francia, Filipe Leal, Susana Montenegro Gouveia, Catarina Nabais, Paulo Duarte, Samuel Gilberto, Daniela Brito, Tyler Moyer, Steffi Kandels-Lewis, Midori Ohta, Daiju Kitagawa, Andrew J. Holland, Eric Karsenti, Thierry Lorca, Mariana Lince-Faria, Mónica Bettencourt-Dias, CDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesis, Current Biology, Volume 26, Issue 9, 2016, Pages 1127-1137, ISSN 0960-9822, https://doi.org/10.1016/j.cub.2016.03.055. (http://www.sciencedirect.com/science/article/pii/S0960982216303001)pt_PT
dc.identifier.doi10.1016/j.cub.2016.03.055pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/831
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationControl of Centriole Structure And Number
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0960982216303001?via%3Dihubpt_PT
dc.subjectPLK4pt_PT
dc.subjectCDKpt_PT
dc.subjectSTILpt_PT
dc.subjectmitosispt_PT
dc.subjectcentriole duplicationpt_PT
dc.subjectlicensingpt_PT
dc.subjectcentrosomept_PT
dc.titleCDK1 Prevents Unscheduled PLK4-STIL Complex Assembly in Centriole Biogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleControl of Centriole Structure And Number
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIM-ONC%2F0830%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/261344/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F105616%2F2008/PT
oaire.citation.endPage1137pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1127pt_PT
oaire.citation.titleCurrent Biologypt_PT
oaire.citation.volume26pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication636d4ec1-0bc6-4977-bd56-06898fff3740
relation.isProjectOfPublication0faa6bb2-99b3-4582-9b24-873d528eceb3
relation.isProjectOfPublication1848efe2-6ac0-4b9c-a402-c3138611e8d1
relation.isProjectOfPublication.latestForDiscovery0faa6bb2-99b3-4582-9b24-873d528eceb3

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