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Natural Genome Diversity of AI-2 Quorum Sensing in Escherichia coli: Conserved Signal Production but Labile Signal Reception

2012-12-10Journal article Open access
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dc.contributor.authorBrito, P. H.
dc.contributor.authorRocha, E. P. C.
dc.contributor.authorXavier, K. B.
dc.contributor.authorGordo, I.
dc.date.accessioned2015-10-23T11:53:14Z
dc.date.available2015-10-23T11:53:14Z
dc.date.issued2012-12-10
dc.description.abstractQuorum sensing (QS) regulates the onset of bacterial social responses in function to cell density having an important impact in virulence. Autoinducer-2 (AI-2) is a signal that has the peculiarity of mediating both intra- and interspecies bacterial QS. We analyzed the diversity of all components of AI-2 QS across 44 complete genomes of Escherichia coli and Shigella strains. We used phylogenetic tools to study its evolution and determined the phenotypes of single-deletion mutants to predict phenotypes of natural strains. Our analysis revealed many likely adaptive polymorphisms both in gene content and in nucleotide sequence. We show that all natural strains possess the signal emitter (the luxS gene), but many lack a functional signal receptor (complete lsr operon) and the ability to regulate extracellular signal concentrations. This result is in striking contrast with the canonical species-specific QS systems where one often finds orphan receptors, without a cognate synthase, but not orphan emitters. Our analysis indicates that selection actively maintains a balanced polymorphism for the presence/absence of a functional lsr operon suggesting diversifying selection on the regulation of signal accumulation and recognition. These results can be explained either by niche-specific adaptation or by selection for a coercive behavior where signal-blind emitters benefit from forcing other individuals in the population to haste in cooperative behaviors.pt_PT
dc.description.sponsorshipInternational Early Career Scientist grant from the Howard Hughes Medical Institute: (HHMI 55007436), Institut Pasteur, the CNRS, FCT award: (SFRH/BPD/26852/2006), salary support of LAO/ITQB & FCT.pt_PT
dc.identifier10.1093/gbe/evs122
dc.identifier.citationPatrícia H. Brito, Eduardo P.C. Rocha, Karina B. Xavier, and Isabel Gordo Natural Genome Diversity of AI-2 Quorum Sensing in Escherichia coli: Conserved Signal Production but Labile Signal Reception Genome Biol Evol (2013) Vol. 5 16-30 first published online December 16, 2012 doi:10.1093/gbe/evs122pt_PT
dc.identifier.doi10.1093/gbe/evs122
dc.identifier.doi10.1093/gbe/evs122
dc.identifier.urihttp://hdl.handle.net/10400.7/434
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationMicrobial adaptation within ecosystems
dc.relationEvolution of gene mobility: how mobile elements shape the function and sociality of microbial communities
dc.relation.publisherversionhttp://gbe.oxfordjournals.org/content/5/1/16.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectgenome evolutionpt_PT
dc.subjectgene losspt_PT
dc.subjectE. colipt_PT
dc.subjectbalancing selectionpt_PT
dc.subjectsocial cheaterpt_PT
dc.subjectbacteria signalingpt_PT
dc.titleNatural Genome Diversity of AI-2 Quorum Sensing in Escherichia coli: Conserved Signal Production but Labile Signal Receptionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardTitleEvolution of gene mobility: how mobile elements shape the function and sociality of microbial communities
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/281605/EU
oaire.citation.endPage30pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage16pt_PT
oaire.citation.titleGenome Biology and Evolutionpt_PT
oaire.citation.volume5pt_PT
oaire.fundingStreamFP7
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublicationafedb097-aea6-4cea-b2e2-835ef0e8ea1f
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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