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The Trk Potassium Transporter Is Required for RsmB-Mediated Activation of Virulence in the Phytopathogen Pectobacterium wasabiae

2016Journal article Open access
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dc.contributor.authorValente, Rita S.
dc.contributor.authorXavier, Karina Bivar
dc.date.accessioned2020-04-06T12:56:46Z
dc.date.available2020-04-06T12:56:46Z
dc.date.issued2016
dc.description.abstractPectobacterium wasabiae (previously known as Erwinia carotovora) is an important plant pathogen that regulates the production of plant cell wall-degrading enzymes through an N-acyl homoserine lactone-based quorum sensing system and through the GacS/GacA two-component system (also known as ExpS/ExpA). At high cell density, activation of GacS/GacA induces the expression of RsmB, a noncoding RNA that is essential for the activation of virulence in this bacterium. A genetic screen to identify regulators of RsmB revealed that mutants defective in components of a putative Trk potassium transporter (trkH and trkA) had decreased rsmB expression. Further analysis of these mutants showed that changes in potassium concentration influenced rsmB expression and consequent tissue damage in potato tubers and that this regulation required an intact Trk system. Regulation of rsmB expression by potassium via the Trk system occurred even in the absence of the GacS/GacA system, demonstrating that these systems act independently and are both required for full activation of RsmB and for the downstream induction of virulence in potato infection assays. Overall, our results identified potassium as an essential environmental factor regulating the Rsm system, and the consequent induction of virulence, in the plant pathogen P. wasabiae.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1128/JB.00569-15pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/951
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relationFCTpt_PT
dc.relation.publisherversionhttps://jb.asm.org/content/198/2/248.longpt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectCarrier Proteinspt_PT
dc.subjectGene Expression Regulation, Bacterialpt_PT
dc.subjectMutationpt_PT
dc.subjectPectobacteriumpt_PT
dc.subjectPotassiumpt_PT
dc.subjectTranscription Factorspt_PT
dc.titleThe Trk Potassium Transporter Is Required for RsmB-Mediated Activation of Virulence in the Phytopathogen Pectobacterium wasabiaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage255pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage248pt_PT
oaire.citation.titleJournal of Bacteriologypt_PT
oaire.citation.volume198pt_PT
person.familyNameXavier
person.givenNameKarina
person.identifier.ciencia-id641F-7328-B863
person.identifier.orcid0000-0001-5210-1434
person.identifier.ridB-9425-2008
person.identifier.scopus-author-id6603926993
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication26248334-983e-47c9-b1af-769585eec81b
relation.isAuthorOfPublication.latestForDiscovery26248334-983e-47c9-b1af-769585eec81b

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