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Sialoglycoproteins and N-Glycans from Secreted Exosomes of Ovarian Carcinoma Cells

2013-10-24Journal article Open access
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dc.contributor.authorEscrevente, Cristina
dc.contributor.authorGrammel, Nicolas
dc.contributor.authorKandzia, Sebastian
dc.contributor.authorZeiser, Johannes
dc.contributor.authorTranfield, Erin M.
dc.contributor.authorConradt, Harald S.
dc.contributor.authorCosta, Júlia
dc.date.accessioned2015-10-28T09:56:57Z
dc.date.available2015-10-28T09:56:57Z
dc.date.issued2013-10-24
dc.description.abstractExosomes consist of vesicles that are secreted by several human cells, including tumor cells and neurons, and they are found in several biological fluids. Exosomes have characteristic protein and lipid composition, however, the results concerning glycoprotein composition and glycosylation are scarce. Here, protein glycosylation of exosomes from ovarian carcinoma SKOV3 cells has been studied by lectin blotting, NP-HPLC analysis of 2-aminobenzamide labeled glycans and mass spectrometry. An abundant sialoglycoprotein was found enriched in exosomes and it was identified by peptide mass fingerprinting and immunoblot as the galectin-3-binding protein (LGALS3BP). Exosomes were found to contain predominantly complex glycans of the di-, tri-, and tetraantennary type with or without proximal fucose and also high mannose glycans. Diantennary glycans containing bisecting N-acetylglucosamine were also detected. This work provides detailed information about glycoprotein and N-glycan composition of exosomes from ovarian cancer cells, furthermore it opens novel perspectives to further explore the functional role of glycans in the biology of exosomes.pt_PT
dc.description.sponsorshipEU Joint Programme JPND/0003/2011, FCT grant: Pest-OE/EQB/LA0004/2011, FCT PhD fellowship.pt_PT
dc.identifier10.1371/journal.pone.0078631
dc.identifier.citationEscrevente C, Grammel N, Kandzia S, Zeiser J, Tranfield EM, et al. (2013) Sialoglycoproteins and N-Glycans from Secreted Exosomes of Ovarian Carcinoma Cells. PLoS ONE 8(10): e78631. doi:10.1371/journal.pone.0078631pt_PT
dc.identifier.doi10.1371/journal.pone.0078631
dc.identifier.urihttp://hdl.handle.net/10400.7/447
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relation.publisherversionhttp://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0078631pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSialoglycoproteinspt_PT
dc.subjectExosomespt_PT
dc.subjectOvarian Neoplasmspt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectTumor Markers, Biologicalpt_PT
dc.titleSialoglycoproteins and N-Glycans from Secreted Exosomes of Ovarian Carcinoma Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-NEU%2F100724%2F2008/PT
oaire.citation.endPage11pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLOS Onept_PT
oaire.citation.volume8pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication5482eba8-a8ab-4d7f-a443-72a44741bfc7
relation.isProjectOfPublication.latestForDiscovery5482eba8-a8ab-4d7f-a443-72a44741bfc7

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