dc.contributor.author	Valente, Rita S.
dc.contributor.author	Nadal-Jimenez, Pol
dc.contributor.author	Carvalho, André F. P.
dc.contributor.author	Vieira, Filipe J. D.
dc.contributor.author	Xavier, Karina B.
dc.date.accessioned	2017-05-29T11:10:24Z
dc.date.available	2017-05-29T11:10:24Z
dc.date.issued	2017-05-23
dc.description	This deposit is composed by the main article plus the supplementary materials of the publication.	pt_PT
dc.description.abstract	Bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. The diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. In the plant pathogen Pectobacterium wasabiae (formerly Erwinia carotovora), two signaling networks-the N-acyl homoserine lactone (AHL) quorum-sensing system and the Gac/Rsm signal transduction pathway-control the expression of secreted plant cell wall-degrading enzymes, its major virulence determinants. We show that the AHL system controls the Gac/Rsm system by affecting the expression of the regulatory RNA RsmB. This regulation is mediated by ExpR2, the quorum-sensing receptor that responds to the P. wasabiae cognate AHL but also to AHLs produced by other bacterial species. As a consequence, this level of regulation allows P. wasabiae to bypass the Gac-dependent regulation of RsmB in the presence of exogenous AHLs or AHL-producing bacteria. We provide in vivo evidence that this pivotal role of RsmB in signal transduction is important for the ability of P. wasabiae to induce virulence in response to other AHL-producing bacteria in multispecies plant lesions. Our results suggest that the signaling architecture in P. wasabiae was coopted to prime the bacteria to eavesdrop on other bacteria and quickly join the efforts of other species, which are already exploiting host resources.IMPORTANCE Quorum-sensing mechanisms enable bacteria to communicate through small signal molecules and coordinate group behaviors. Often, bacteria have various quorum-sensing receptors and integrate information with other signal transduction pathways, presumably allowing them to respond to different ecological contexts. The plant pathogen Pectobacterium wasabiae has two N-acyl homoserine lactone receptors with apparently the same regulatory functions. Our work revealed that the receptor with the broadest signal specificity is also responsible for establishing the link between the main signaling pathways regulating virulence in P. wasabiae This link is essential to provide P. wasabiae with the ability to induce virulence earlier in response to higher densities of other bacterial species. We further present in vivo evidence that this novel regulatory link enables P. wasabiae to join related bacteria in the effort to degrade host tissue in multispecies plant lesions. Our work provides support for the hypothesis that interspecies interactions are among the major factors influencing the network architectures observed in bacterial quorum-sensing pathways.	pt_PT
dc.description.sponsorship	Fundação Para a Ciência e Tecnologia grants: (PTDC/BIA-BCM/101585/2008, SFRH/BD/33570/2008, SRFH/BD/113986/2015, PD/00133/2012); Marie Curie Intra-European grant: (PIEF-GA-2011-301365); Howard Hughes Medical Institute international early career scientist grant: (HHMI 55007436).	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Valente RS, Nadal-Jimenez P, Carvalho AFP, Vieira FJD, Xavier KB. 2017. Signal integration in quorum sensing enables cross- species induction of virulence in Pectobacterium wasabiae. mBio 8:e00398-17. https://doi.org/10.1128/mBio.00398-17 .	pt_PT
dc.identifier.doi	10.1128/mBio.00398-17	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/761
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	American Society for Microbiology	pt_PT
dc.relation	SRFH/BD/113986/2015	pt_PT
dc.relation	Molecular mechanisms in the establishment of disease transmission by invertebrate vectors
dc.relation	Inhibition of bacterial plant virulence by interference with interspecies cell-cell communication.
dc.relation.publisherversion	http://mbio.asm.org/content/8/3/e00398-17.long	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Bacterial	pt_PT
dc.subject	Pectobacterium wasabiae	pt_PT
dc.subject	ExpR2	pt_PT
dc.subject	AHL system	pt_PT
dc.subject	Virulence	pt_PT
dc.subject	N-Acyl homoserine lactone	pt_PT
dc.subject	RNA RsmB	pt_PT
dc.subject	Gac/Rsm	pt_PT
dc.title	Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	Molecular mechanisms in the establishment of disease transmission by invertebrate vectors
oaire.awardTitle	Inhibition of bacterial plant virulence by interference with interspecies cell-cell communication.
oaire.awardURI	info:eu-repo/grantAgreement/EC/FP7/301365/EU
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F101585%2F2008/PT
oaire.citation.endPage	16	pt_PT
oaire.citation.issue	3	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	mBio	pt_PT
oaire.citation.volume	8	pt_PT
oaire.fundingStream	FP7
oaire.fundingStream	3599-PPCDT
project.funder.identifier	http://doi.org/10.13039/501100008530
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	European Commission
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	16a1338b-2d6d-416a-85bc-f6e6acc2b6c1
relation.isProjectOfPublication	7ff19085-e9cd-4a5a-8a35-9e665f495c19
relation.isProjectOfPublication.latestForDiscovery	16a1338b-2d6d-416a-85bc-f6e6acc2b6c1

Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

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