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The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules

2001-12Journal article Restricted access
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dc.contributor.authorBecker, J.D.
dc.contributor.authorMoreira, L.M.
dc.contributor.authorKapp, D.
dc.contributor.authorFrosch S.C.
dc.contributor.authorPühler A.
dc.contributor.authorPerlick, A.M.
dc.date.accessioned2010-03-19T11:29:46Z
dc.date.available2010-03-19T11:29:46Z
dc.date.issued2001-12
dc.description.abstractRecently we described the novel nodulin gene VfENOD18, whose corresponding transcripts were restricted to the nitrogen-fixing zone III of broad bean root nodules. To characterize VfENOD18 on the protein level, polyclonal antibodies were generated using the purified recombinant VfENOD18 protein produced in Escherichia coli by employing the pMAL-c expression system. These antibodies recognized immunoreactive proteins isolated from indeterminate nodules of different leguminous plants, but also from non-symbiotic tissues of Glycine max and from tissues of Arabidopsis thaliana and Zea mays. Using immunogold labelling the nodulin VfENOD18 was localized to the cytoplasm of infected cells in the nitrogen-fixing zone of broad bean nodules. Due to the homology of the VfENOD18 sequence to that of the ATP-binding protein MJ0577 from the hyperthermophile Methanococcus jannaschii the recombinant VfENOD18 protein was tested for ATP-binding. Using the biotin photoaffinity ATP analogue 8N3ATP[gamma]biotin it could be demonstrated that VfENOD18 is an ATP-binding protein. PCR experiments revealed that the amino acid sequences of the putative C-terminal ATP-binding sites of the VfENOD 18 homologues from Lens culinaris, Vicia hirsuta, Vicia sativa and Vicia villosa were conserved. We propose that VfENOD18 is a member of a novel family of ATP-binding proteins in plants.
dc.identifier.citationBecker, J.D, Moreira LM, Kapp D, Frosch SC, Pühler A, Perlick AM (2001). The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. Plant Molecular Biology. 47 :749-759
dc.identifier.issnISSN:0167-4412
dc.identifier.urihttp://hdl.handle.net/10400.7/74
dc.identifier.urihttp://dx.doi.org/10.1023/A:1013664311052
dc.language.isoengpt
dc.publisherSpringer
dc.subjectAdenosine Triphosphate/metabolism
dc.subjectFabaceae/cytology/genetics/microbiology
dc.subjectMembrane Proteins
dc.subjectPlant Proteins/genetics/immunology/metabolism
dc.subjectPlant Roots/genetics/microbiology/ultrastructure
dc.subjectbroad bean
dc.subjectheterologous expression
dc.subjectMJ0577 superfamily
dc.subjectnitrogen fixation
dc.subjectnodule-specific gene expression
dc.titleThe nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodulespt
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage759pt
oaire.citation.issue47pt
oaire.citation.startPage749pt
oaire.citation.titlePlant Molecular Biologypt
rcaap.rightsopenAccesspt
rcaap.typearticlept

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