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Distinct mechanisms eliminate mother and daughter centrioles in meiosis of starfish oocytes

2016-03-21Journal article Open access
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dc.contributor.authorBorrego-Pinto, Joana
dc.contributor.authorSomogyi, Kálmán
dc.contributor.authorKarreman, Matthia A.
dc.contributor.authorKönig, Julia
dc.contributor.authorMüller-Reichert, Thomas
dc.contributor.authorBettencourt-Dias, Mónica
dc.contributor.authorGönczy, Pierre
dc.contributor.authorSchwab, Yannick
dc.contributor.authorLénárt, Péter
dc.date.accessioned2016-06-09T12:07:35Z
dc.date.available2016-09-21T00:30:08Z
dc.date.issued2016-03-21
dc.description.abstractCentriole elimination is an essential process that occurs in female meiosis of metazoa to reset centriole number in the zygote at fertilization. How centrioles are eliminated remains poorly understood. Here we visualize the entire elimination process live in starfish oocytes. Using specific fluorescent markers, we demonstrate that the two older, mother centrioles are selectively removed from the oocyte by extrusion into polar bodies. We show that this requires specific positioning of the second meiotic spindle, achieved by dynein-driven transport, and anchorage of the mother centriole to the plasma membrane via mother-specific appendages. In contrast, the single daughter centriole remaining in the egg is eliminated before the first embryonic cleavage. We demonstrate that these distinct elimination mechanisms are necessary because if mother centrioles are artificially retained, they cannot be inactivated, resulting in multipolar zygotic spindles. Thus, our findings reveal a dual mechanism to eliminate centrioles: mothers are physically removed, whereas daughters are eliminated in the cytoplasm, preparing the egg for fertilization.pt_PT
dc.description.sponsorshipEuropean Molecular Biology Laboratory (EMBL)- EMBL International PhD Program; Laura and Arthur Colwin Endowed Summer Research Fellowship; Deutsche Forschungsgemeinschaft grant: (MU1423/4-1).pt_PT
dc.identifier.citationDistinct mechanisms eliminate mother and daughter centrioles in meiosis of starfish oocytes Joana Borrego-Pinto, Kálmán Somogyi, Matthia A. Karreman, Julia König, Thomas Müller-Reichert, Mónica Bettencourt-Dias, Pierre Gönczy, Yannick Schwab, and Péter Lénárt J Cell Biol 2016 212:815-827. Published March 21, 2016, doi:10.1083/jcb.201510083pt_PT
dc.identifier.doi10.1083/jcb.201510083pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/635
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherThe Rockefeller University Presspt_PT
dc.relation.publisherversionhttp://jcb.rupress.org/content/212/7/815.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectCentriolespt_PT
dc.subjectOocytespt_PT
dc.subjectMeiosispt_PT
dc.subjectFertilizationpt_PT
dc.titleDistinct mechanisms eliminate mother and daughter centrioles in meiosis of starfish oocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage827pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage815pt_PT
oaire.citation.titleThe Journal of Cell Biologypt_PT
oaire.citation.volume212pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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