Transcriptional profiling in Saccharomyces cerevisiae relevant for predicting alachlor mechanisms of toxicity

Gil, Fátima N.; Gonçalves, Alina C.; Jacinto, Maria João; Becker, Jörg D.; Viegas, Cristina A.

Publication

Transcriptional profiling in Saccharomyces cerevisiae relevant for predicting alachlor mechanisms of toxicity

2011-10-14Journal article

dc.contributor.author	Gil, Fátima N.
dc.contributor.author	Gonçalves, Alina C.
dc.contributor.author	Jacinto, Maria João
dc.contributor.author	Becker, Jörg D.
dc.contributor.author	Viegas, Cristina A.
dc.date.accessioned	2016-11-22T15:30:26Z
dc.date.available	2016-11-22T15:30:26Z
dc.date.issued	2011-10-14
dc.description.abstract	Alachlor has been a commonly applied herbicide and is a substance of ecotoxicological concern. The present study aims to identify molecular biomarkers in the eukaryotic model Saccharomyces cerevisiae that can be used to predict potential cytotoxic effects of alachlor, while providing new mechanistic clues with possible relevance for experimentally less accessible eukaryotes. It focuses on genome-wide expression profiling in a yeast population in response to two exposure scenarios exerting effects from slight to moderate magnitude at phenotypic level. In particular, 100 and 264 genes, respectively, were found as differentially expressed on a 2-h exposure of yeast cells to the lowest observed effect concentration (110 mg/L) and the 20% inhibitory concentration (200 mg/L) of alachlor, in comparison with cells not exposed to the herbicide. The datasets of alachlor-responsive genes showed functional enrichment in diverse metabolic, transmembrane transport, cell defense, and detoxification categories. In general, the modifications in transcript levels of selected candidate biomarkers, assessed by quantitative reverse transcriptase polymerase chain reaction, confirmed the microarray data and varied consistently with the growth inhibitory effects of alachlor. Approximately 16% of the proteins encoded by alachlor-differentially expressed genes were found to share significant homology with proteins from ecologically relevant eukaryotic species. The biological relevance of these results is discussed in relation to new insights into the potential adverse effects of alachlor in health of organisms from ecosystems, particularly in worst-case situations such as accidental spills or careless storage, usage, and disposal.	pt_PT
dc.description.sponsorship	Fundo Europeu de Desenvolvimento Regional; Fundação para a Ciência e Tecnologia BII fellowship.	pt_PT
dc.identifier.citation	Gil, F. N., Gonçalves, A. C., Jacinto, M. J., Becker, J. D. and Viegas, C. A. (2011), Transcriptional profiling in Saccharomyces cerevisiae relevant for predicting alachlor mechanisms of toxicity. Environmental Toxicology and Chemistry, 30: 2506–2518. doi:10.1002/etc.640	pt_PT
dc.identifier.doi	10.1002/etc.640	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.7/719
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Wiley	pt_PT
dc.relation	IDENTIFICATION OF MOLECULAR BIOMARKERS RELEVANT FOR ENVIRONMENTAL BIOMONITORING OF PESTICIDE TOXICITY USING SIMPLE EUKARYOTIC MODELS
dc.relation.publisherversion	http://onlinelibrary.wiley.com/doi/10.1002/etc.640/abstract	pt_PT
dc.subject	Acetamides	pt_PT
dc.subject	Gene Expression Profiling	pt_PT
dc.subject	Herbicides	pt_PT
dc.subject	Oligonucleotide Array Sequence Analysis	pt_PT
dc.subject	Reverse Transcriptase Polymerase Chain Reaction	pt_PT
dc.subject	Saccharomyces cerevisiae	pt_PT
dc.subject	Saccharomyces cerevisiae Proteins	pt_PT
dc.subject	Transcriptome	pt_PT
dc.title	Transcriptional profiling in Saccharomyces cerevisiae relevant for predicting alachlor mechanisms of toxicity	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	IDENTIFICATION OF MOLECULAR BIOMARKERS RELEVANT FOR ENVIRONMENTAL BIOMONITORING OF PESTICIDE TOXICITY USING SIMPLE EUKARYOTIC MODELS
oaire.awardURI	info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAMB%2F64230%2F2006/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F60933%2F2009/PT
oaire.citation.endPage	2518	pt_PT
oaire.citation.issue	11	pt_PT
oaire.citation.startPage	2506	pt_PT
oaire.citation.title	Environmental Toxicology and Chemistry	pt_PT
oaire.citation.volume	30	pt_PT
oaire.fundingStream	3599-PPCDT
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	restrictedAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	fc86bd6d-a572-4c39-a570-bc49882b05b2
relation.isProjectOfPublication	90b611cd-2b1d-43da-84a9-8d399244318a
relation.isProjectOfPublication.latestForDiscovery	90b611cd-2b1d-43da-84a9-8d399244318a