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Experimental determination of invasive fitness in Caenorhabditis elegans
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| main article | 539.11 KB | Adobe PDF |
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Orientador(es)
Resumo(s)
Estimation of fitness is a key step in experimental evolution studies. However, no established methods currently exist to specifically estimate how successful new alleles are in invading populations. The main reason is that most assays do not accurately reflect the randomness associated with the first stages of the invasion, when invaders are rare and extinctions are frequent. In this protocol, I describe how such experiments can be done in an effective way. By using the nematode model, Caenorhabditis elegans, a large number of invasion experiments are set up, whereby invading individuals carrying a visual marker are introduced into populations in very low numbers. The number of invaders counted in consecutive generations, together with the number of extinctions, is then used in the context of individual-based computer simulations to provide likelihood (Lk) estimates for fitness. This protocol can take up to five generations of experimental invasions and a few hours of computer processing time.
Descrição
The deposited article is a post-print version and has been submitted to peer reviewing. This publication hasn't any creative commons license associated. The deposited article version contains attached some supplementary materials within the pdf. Some supplementary materials are not present in the uploaded version of the article, are present in the publisher's page in the following link: https://www.nature.com/articles/nprot.2014.098#integrated-supplementary-information
Palavras-chave
Animals Caenorhabditis elegans Computer Simulation Genetic Fitness Likelihood Functions Alleles Genetics, Population
Contexto Educativo
Citação
Chelo, I. M. Experimental determination of invasive fitness in Caenorhabditis elegans. Nat Protoc 9, 1392–1400 (2014).
Editora
Nature Publishing Group