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Experimental determination of invasive fitness in Caenorhabditis elegans

2014-05-22Journal article Open access
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dc.contributor.authorChelo, Ivo M
dc.date.accessioned2017-11-22T12:34:53Z
dc.date.available2017-11-22T12:34:53Z
dc.date.issued2014-05-22
dc.descriptionThe deposited article is a post-print version and has been submitted to peer reviewing. This publication hasn't any creative commons license associated. The deposited article version contains attached some supplementary materials within the pdf. Some supplementary materials are not present in the uploaded version of the article, are present in the publisher's page in the following link: https://www.nature.com/articles/nprot.2014.098#integrated-supplementary-informationpt_PT
dc.description.abstractEstimation of fitness is a key step in experimental evolution studies. However, no established methods currently exist to specifically estimate how successful new alleles are in invading populations. The main reason is that most assays do not accurately reflect the randomness associated with the first stages of the invasion, when invaders are rare and extinctions are frequent. In this protocol, I describe how such experiments can be done in an effective way. By using the nematode model, Caenorhabditis elegans, a large number of invasion experiments are set up, whereby invading individuals carrying a visual marker are introduced into populations in very low numbers. The number of invaders counted in consecutive generations, together with the number of extinctions, is then used in the context of individual-based computer simulations to provide likelihood (Lk) estimates for fitness. This protocol can take up to five generations of experimental invasions and a few hours of computer processing time.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grant: (EXPL/BIA-EFV/1211/2013); Human Frontiers Science Program grant: (RGP0045/2010).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationChelo, I. M. Experimental determination of invasive fitness in Caenorhabditis elegans. Nat Protoc 9, 1392–1400 (2014).pt_PT
dc.identifier.doi10.1038/nprot.2014.098pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/809
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationRGP00457/2010pt_PT
dc.relationDiversity and frequency-dependent selection in Caenorhabditis elegans
dc.relation.publisherversionhttps://www.nature.com/articles/nprot.2014.098pt_PT
dc.subjectAnimalspt_PT
dc.subjectCaenorhabditis eleganspt_PT
dc.subjectComputer Simulationpt_PT
dc.subjectGenetic Fitnesspt_PT
dc.subjectLikelihood Functionspt_PT
dc.subjectAllelespt_PT
dc.subjectGenetics, Populationpt_PT
dc.titleExperimental determination of invasive fitness in Caenorhabditis eleganspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDiversity and frequency-dependent selection in Caenorhabditis elegans
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-EVF%2F1211%2F2013/PT
oaire.citation.endPage1400pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1392pt_PT
oaire.citation.titleNature Protocolspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication08994d69-f865-483a-83b8-c8678993afcd
relation.isProjectOfPublication.latestForDiscovery08994d69-f865-483a-83b8-c8678993afcd

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