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A Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gut

2016-11-03Journal article Open access
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dc.contributor.authorLourenço, Marta
dc.contributor.authorRamiro, Ricardo S
dc.contributor.authorGüleresi, Daniela
dc.contributor.authorBarroso-Batista, João
dc.contributor.authorXavier, Karina B
dc.contributor.authorGordo, Isabel
dc.contributor.authorSousa, Ana
dc.date.accessioned2016-11-08T16:20:38Z
dc.date.available2016-11-08T16:20:38Z
dc.date.issued2016-11-03
dc.description.abstractThe relative role of drift versus selection underlying the evolution of bacterial species within the gut microbiota remains poorly understood. The large sizes of bacterial populations in this environment suggest that even adaptive mutations with weak effects, thought to be the most frequently occurring, could substantially contribute to a rapid pace of evolutionary change in the gut. We followed the emergence of intra-species diversity in a commensal Escherichia coli strain that previously acquired an adaptive mutation with strong effect during one week of colonization of the mouse gut. Following this first step, which consisted of inactivating a metabolic operon, one third of the subsequent adaptive mutations were found to have a selective effect as high as the first. Nevertheless, the order of the adaptive steps was strongly affected by a mutational hotspot with an exceptionally high mutation rate of 10-5. The pattern of polymorphism emerging in the populations evolving within different hosts was characterized by periodic selection, which reduced diversity, but also frequency-dependent selection, actively maintaining genetic diversity. Furthermore, the continuous emergence of similar phenotypes due to distinct mutations, known as clonal interference, was pervasive. Evolutionary change within the gut is therefore highly repeatable within and across hosts, with adaptive mutations of selection coefficients as strong as 12% accumulating without strong constraints on genetic background. In vivo competitive assays showed that one of the second steps (focA) exhibited positive epistasis with the first, while another (dcuB) exhibited negative epistasis. The data shows that strong effect adaptive mutations continuously recur in gut commensal bacterial species.pt_PT
dc.description.sponsorshipDeutsche Forschungs gemeinschaft grant: (SFB 680); Fundação para a Ciência e a Tecnologia grant: (SFRH/BPD/1117 25/2015).pt_PT
dc.identifier.citationLourenc Ào M, Ramiro RS, Gu»leresi D, Barroso-Ba tista J, Xavier KB, Gordo I, et al. (2016) A Mutational Hotspot and Strong Selection Contribute to the Order of Mutatio ns Selected for during Escherichia coli Adaptation to the Gut. PLoS Genet 12(11): e1006420. doi:10.137 1/journal. pgen.100642 0pt_PT
dc.identifier.doi10.1371/journal.pgen.1006420pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/707
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relationMicrobial adaptation within ecosystems
dc.relationAdaptation of commensal bacteria to the mammalian gut
dc.relation.publisherversionhttp://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006420pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCloningpt_PT
dc.subjectInsertion mutationpt_PT
dc.subjectNonsense mutationpt_PT
dc.subjectNatural selectionpt_PT
dc.subjectDeletion mutationpt_PT
dc.subjectOperonspt_PT
dc.subjectEvolutionary adaptationpt_PT
dc.subjectMicrobial evolutionpt_PT
dc.titleA Mutational Hotspot and Strong Selection Contribute to the Order of Mutations Selected for during Escherichia coli Adaptation to the Gutpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicrobial adaptation within ecosystems
oaire.awardTitleAdaptation of commensal bacteria to the mammalian gut
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/260421/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-EVF%2F118075%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/RECI%2FIMI-IMU%2F0038%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F80257%2F2011/PT
oaire.citation.endPage23pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titlePLoS Geneticspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStreamFP7
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1f01df05-f8af-4e76-8dec-0fc6237b41fd
relation.isProjectOfPublication8a096e46-5521-429a-b9e8-8a7901fe8b30
relation.isProjectOfPublication1470c3e4-1d23-40cc-9500-9f8843b0e35d
relation.isProjectOfPublication826481bc-422c-4287-b03b-1d7903b94c72
relation.isProjectOfPublication.latestForDiscovery1f01df05-f8af-4e76-8dec-0fc6237b41fd

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