Repository logo
 
Publication

Specific Eco-evolutionary Contexts in the Mouse Gut Reveal Escherichia coli Metabolic Versatility

2020-02-03Journal article Open access
Simple item page
dc.contributor.authorBarroso-Batista, João
dc.contributor.authorPedro, Miguel F
dc.contributor.authorSales-Dias, Joana
dc.contributor.authorPinto, Catarina J G
dc.contributor.authorThompson, Jessica A
dc.contributor.authorPereira, Helena
dc.contributor.authorDemengeot, Jocelyne
dc.contributor.authorGordo, Isabel
dc.contributor.authorXavier, Karina Bivar
dc.date.accessioned2020-03-20T12:14:07Z
dc.date.available2020-03-20T12:14:07Z
dc.date.issued2020-02-03
dc.description.abstractMembers of the gut microbiota are thought to experience strong competition for nutrients. However, how such competition shapes their evolutionary dynamics and depends on intra- and interspecies interactions is poorly understood. Here, we test the hypothesis that Escherichia coli evolution in the mouse gut is more predictable across hosts in the absence of interspecies competition than in the presence of other microbial species. In support, we observed that lrp, a gene encoding a global regulator of amino acid metabolism, was repeatedly selected in germ-free mice 2 weeks after mono-colonization by this bacterium. We established that this specific genetic adaptation increased E. coli's ability to compete for amino acids, and analysis of gut metabolites identified serine and threonine as the metabolites preferentially consumed by E. coli in the mono-colonized mouse gut. Preference for serine consumption was further supported by testing a set of mutants that showed loss of advantage of an lrp mutant impaired in serine metabolism in vitro and in vivo. Remarkably, the presence of a single additional member of the microbiota, Blautia coccoides, was sufficient to alter the gut metabolome and, consequently, the evolutionary path of E. coli. In this environment, the fitness advantage of the lrp mutant bacteria is lost, and mutations in genes involved in anaerobic respiration were selected instead, recapitulating the eco-evolutionary context from mice with a complex microbiota. Together, these results highlight the metabolic plasticity and evolutionary versatility of E. coli, tailored to the specific ecology it experiences in the gut.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cub.2020.01.050pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/946
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationFCTpt_PT
dc.relation.publisherversionhttps://www.cell.com/current-biology/fulltext/S0960-9822(20)30094-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982220300944%3Fshowall%3Dtruept_PT
dc.subjectBlautia coccoidespt_PT
dc.subjectEcherichia colipt_PT
dc.subjectamino acid metabolismpt_PT
dc.subjectserine metabolismpt_PT
dc.subjectlrppt_PT
dc.subjectmetabolomicspt_PT
dc.subjectgutpt_PT
dc.subjectgnotobiologypt_PT
dc.subjectgerm-freept_PT
dc.subjectevolutionpt_PT
dc.titleSpecific Eco-evolutionary Contexts in the Mouse Gut Reveal Escherichia coli Metabolic Versatilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleCurrent biology : CBpt_PT
person.familyNameXavier
person.givenNameKarina
person.identifier.ciencia-id641F-7328-B863
person.identifier.orcid0000-0001-5210-1434
person.identifier.ridB-9425-2008
person.identifier.scopus-author-id6603926993
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication26248334-983e-47c9-b1af-769585eec81b
relation.isAuthorOfPublication.latestForDiscovery26248334-983e-47c9-b1af-769585eec81b

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
SSRN-id3433809 (3)_PRE_Print_Barroso_etal_2020.pdf
Size:
4.26 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

BS - Artigos