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Whole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsis

2011-04Journal article Open access
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dc.contributor.authorBoavida, L. C.
dc.contributor.authorBorges, F.
dc.contributor.authorBecker, J. D.
dc.contributor.authorFeijo, J. A.
dc.date.accessioned2016-10-18T16:02:41Z
dc.date.available2016-10-18T16:02:41Z
dc.date.issued2011-04
dc.description.abstractPlant reproduction depends on the concerted activation of many genes to ensure correct communication between pollen and pistil. Here, we queried the whole transcriptome of Arabidopsis (Arabidopsis thaliana) in order to identify genes with specific reproductive functions. We used the Affymetrix ATH1 whole genome array to profile wild-type unpollinated pistils and unfertilized ovules. By comparing the expression profile of pistils at 0.5, 3.5, and 8.0 h after pollination and applying a number of statistical and bioinformatics criteria, we found 1,373 genes differentially regulated during pollen-pistil interactions. Robust clustering analysis grouped these genes in 16 time-course clusters representing distinct patterns of regulation. Coregulation within each cluster suggests the presence of distinct genetic pathways, which might be under the control of specific transcriptional regulators. A total of 78% of the regulated genes were expressed initially in unpollinated pistil and/or ovules, 15% were initially detected in the pollen data sets as enriched or preferentially expressed, and 7% were induced upon pollination. Among those, we found a particular enrichment for unknown transcripts predicted to encode secreted proteins or representing signaling and cell wall-related proteins, which may function by remodeling the extracellular matrix or as extracellular signaling molecules. A strict regulatory control in various metabolic pathways suggests that fine-tuning of the biochemical and physiological cellular environment is crucial for reproductive success. Our study provides a unique and detailed temporal and spatial gene expression profile of in vivo pollen-pistil interactions, providing a framework to better understand the basis of the molecular mechanisms operating during the reproductive process in higher plants.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia grants: (FCT–PTDC/QUI/64339/06, SFRH/BD/1128/2000).pt_PT
dc.identifier.citationLeonor C. Boavida, Filipe Borges, Jörg D. Becker, and José A. Feijó Whole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsis Plant Physiol. 2011 155: 2066-2080. First Published on February 11, 2011; doi:10.1104/pp.110.169813pt_PT
dc.identifier.doi10.1104/pp.110.169813pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.7/703
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society of Plant Biologistspt_PT
dc.relationOscillatory behavior in pollen tubes: the biophysical and biochemical fundamental basis of cell growth and morphogenesis
dc.relationGENETIC AND EPIGENETIC MECHANISMS REGULATING SPERM CELL FUNCTIONS DURING DOUBLE FERTILIZATION IN FLOWERING PLANTS
dc.relationGENECHIP ANALYSIS OF THE ARABIDOPSIS SPERM CELL TRANSCRIPTOME AND POLLEN SMALL RNAS
dc.relation.publisherversionhttp://www.plantphysiol.org/content/155/4/2066.longpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectArabidopsispt_PT
dc.subjectCluster Analysispt_PT
dc.subjectComputational Biologypt_PT
dc.subjectFlowerspt_PT
dc.subjectGene Expression Regulation, Developmentalpt_PT
dc.subjectGene Expression Regulation, Plantpt_PT
dc.subjectGenome, Plantpt_PT
dc.subjectOligonucleotide Array Sequence Analysispt_PT
dc.subjectPollenpt_PT
dc.subjectPollinationpt_PT
dc.subjectRNA, Plantpt_PT
dc.subjectTime Factorspt_PT
dc.subjectGene Expression Profilingpt_PT
dc.subjectMetabolic Networks and Pathwayspt_PT
dc.subjectSignal Transductionpt_PT
dc.titleWhole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleOscillatory behavior in pollen tubes: the biophysical and biochemical fundamental basis of cell growth and morphogenesis
oaire.awardTitleGENETIC AND EPIGENETIC MECHANISMS REGULATING SPERM CELL FUNCTIONS DURING DOUBLE FERTILIZATION IN FLOWERING PLANTS
oaire.awardTitleGENECHIP ANALYSIS OF THE ARABIDOPSIS SPERM CELL TRANSCRIPTOME AND POLLEN SMALL RNAS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-BCM%2F60046%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PPCDT%2FBIA-BCM%2F61270%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-BCM%2F108044%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F48761%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F31047%2F2006/PT
oaire.citation.endPage2080pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage2066pt_PT
oaire.citation.titlePlant Physiologypt_PT
oaire.citation.volume155pt_PT
oaire.fundingStreamPOCI
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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